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H3K27me3

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K27me3
Vendor: EpiCypher Catalog #: 13-0030 Lot #: 18303001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0777.1
10.11%
3.71
Unmodified
3.81
H3K4me1
3.75
H3K4me2
4.40
H3K4me3
3.84
H3K9me1
3.85
H3K9me2
3.35
H3K9me3
4.74
H3K27me1
5.34
H3K27me2
100.00
H3K27me3
4.32
H3K36me1
5.15
H3K36me2
10.55
H3K36me3
3.37
H4K20me1
3.48
H4K20me2
5.93
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TE2567832 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.2
12.07%
7.22
Unmodified
4.26
H3K4me1
6.67
H3K4me2
9.56
H3K4me3
7.29
H3K9me1
4.68
H3K9me2
3.93
H3K9me3
6.21
H3K27me1
6.18
H3K27me2
100.00
H3K27me3
3.69
H3K36me1
6.54
H3K36me2
5.97
H3K36me3
1.94
H4K20me1
8.14
H4K20me2
7.52
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9733 Lot #: 14 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 409
E0332.2
15.06%
9.89
Unmodified
4.78
H3K4me1
9.55
H3K4me2
8.90
H3K4me3
8.51
H3K9me1
5.88
H3K9me2
4.56
H3K9me3
7.05
H3K27me1
7.25
H3K27me2
100.00
H3K27me3
3.33
H3K36me1
7.59
H3K36me2
7.94
H3K36me3
2.43
H4K20me1
7.73
H4K20me2
8.70
H4K20me3
Vendor: RevMAb Catalog #: 31-1062-00 Lot #: Q-06-01079 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0037.1
9.39%
4.71
Unmodified
4.88
H3K4me1
6.60
H3K4me2
6.54
H3K4me3
6.53
H3K9me1
6.27
H3K9me2
5.46
H3K9me3
11.03
H3K27me1
5.30
H3K27me2
100.00
H3K27me3
10.33
H3K36me1
9.31
H3K36me2
15.29
H3K36me3
6.05
H4K20me1
7.86
H4K20me2
8.09
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TF2589871 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.4
18.63%
10.93
Unmodified
5.73
H3K4me1
10.02
H3K4me2
12.50
H3K4me3
9.87
H3K9me1
6.82
H3K9me2
5.19
H3K9me3
8.26
H3K27me1
8.41
H3K27me2
100.00
H3K27me3
3.89
H3K36me1
8.74
H3K36me2
7.40
H3K36me3
2.64
H4K20me1
10.37
H4K20me2
11.82
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: SF2407175 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.1
6.92%
5.96
Unmodified
11.68
H3K4me1
9.12
H3K4me2
13.88
H3K4me3
6.99
H3K9me1
11.57
H3K9me2
6.99
H3K9me3
9.48
H3K27me1
4.83
H3K27me2
100.00
H3K27me3
11.46
H3K36me1
7.40
H3K36me2
12.27
H3K36me3
10.22
H4K20me1
10.78
H4K20me2
6.45
H4K20me3
Vendor: Active Motif Catalog #: 39055 Lot #: 31618020 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0683.1
3.73%
2.46
Unmodified
1.95
H3K4me1
3.09
H3K4me2
3.92
H3K4me3
2.47
H3K9me1
1.78
H3K9me2
4.65
H3K9me3
3.12
H3K27me1
3.02
H3K27me2
100.00
H3K27me3
2.53
H3K36me1
2.52
H3K36me2
3.41
H3K36me3
1.54
H4K20me1
2.14
H4K20me2
3.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TF2586052 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0045.3
2.07%
2.64
Unmodified
3.73
H3K4me1
2.88
H3K4me2
2.67
H3K4me3
3.71
H3K9me1
3.19
H3K9me2
2.40
H3K9me3
4.09
H3K27me1
3.41
H3K27me2
100.00
H3K27me3
3.41
H3K36me1
4.61
H3K36me2
4.60
H3K36me3
2.90
H4K20me1
3.29
H4K20me2
2.98
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9733 Lot #: 8 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 409
E0332.1
0.78%
3.05
Unmodified
4.48
H3K4me1
3.47
H3K4me2
3.25
H3K4me3
5.64
H3K9me1
3.75
H3K9me2
3.01
H3K9me3
5.41
H3K27me1
4.79
H3K27me2
100.00
H3K27me3
4.31
H3K36me1
5.58
H3K36me2
4.85
H3K36me3
3.26
H4K20me1
3.81
H4K20me2
3.72
H4K20me3
Vendor: Abcam Catalog #: ab192985 Lot #: GR3264827-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 15
E0760.1
4.21%
7.83
Unmodified
5.40
H3K4me1
8.98
H3K4me2
8.97
H3K4me3
8.44
H3K9me1
7.06
H3K9me2
7.45
H3K9me3
9.63
H3K27me1
22.10
H3K27me2
100.00
H3K27me3
5.17
H3K36me1
8.83
H3K36me2
7.26
H3K36me3
3.97
H4K20me1
8.61
H4K20me2
8.15
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33081 Lot #: VC2972315 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0649.2
25.45%
9.04
Unmodified
7.41
H3K4me1
10.78
H3K4me2
19.90
H3K4me3
8.03
H3K9me1
7.46
H3K9me2
14.07
H3K9me3
9.67
H3K27me1
13.98
H3K27me2
100.00
H3K27me3
9.38
H3K36me1
11.27
H3K36me2
17.10
H3K36me3
5.97
H4K20me1
6.99
H4K20me2
25.92
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33081 Lot #: UG2810495 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0649.1
35.38%
11.40
Unmodified
5.09
H3K4me1
11.10
H3K4me2
25.52
H3K4me3
8.88
H3K9me1
7.13
H3K9me2
12.89
H3K9me3
9.35
H3K27me1
16.32
H3K27me2
100.00
H3K27me3
6.61
H3K36me1
9.73
H3K36me2
23.80
H3K36me3
4.49
H4K20me1
10.05
H4K20me2
31.87
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: A15024 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0379.2
1.35%
14.85
Unmodified
10.72
H3K4me1
20.47
H3K4me2
17.64
H3K4me3
13.10
H3K9me1
9.14
H3K9me2
9.50
H3K9me3
28.38
H3K27me1
12.71
H3K27me2
100.00
H3K27me3
15.96
H3K36me1
14.53
H3K36me2
17.79
H3K36me3
10.13
H4K20me1
18.98
H4K20me2
9.68
H4K20me3
Vendor: Abcam Catalog #: ab6002 Lot #: GR3232152-1 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 653
E0478.1
4.35%
59.41
Unmodified
15.17
H3K4me1
39.45
H3K4me2
51.73
H3K4me3
45.98
H3K9me1
27.04
H3K9me2
12.78
H3K9me3
30.97
H3K27me1
53.62
H3K27me2
100.00
H3K27me3
7.48
H3K36me1
46.53
H3K36me2
27.45
H3K36me3
6.20
H4K20me1
43.96
H4K20me2
56.19
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: A15024 Lot #: A1821D Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 2
E0379.1
8.44%
7.07
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
9.29
H3K27me1
6.73
H3K27me2
100.00
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abclonal Catalog #: A2363 Lot #: 1561S Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 13
E0041.1
17.11%
13.78
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
17.27
H3K27me1
10.12
H3K27me2
100.00
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K27me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: SF2407175 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.1
0.00
Unmodified
0.00
H3K4me1
0.10
H3K4me2
0.00
H3K4me3
0.00
H3K9me1
0.00
H3K9me2
0.00
H3K9me3
0.00
H3K27me1
0.10
H3K27me2
100.00
H3K27me3
0.00
H3K36me1
0.10
H3K36me2
0.00
H3K36me3
0.00
H4K20me1
0.10
H4K20me2
0.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TF2586052 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.3
0.10
Unmodified
0.00
H3K4me1
0.00
H3K4me2
0.10
H3K4me3
0.00
H3K9me1
0.00
H3K9me2
0.10
H3K9me3
0.00
H3K27me1
0.00
H3K27me2
100.00
H3K27me3
0.00
H3K36me1
0.00
H3K36me2
0.00
H3K36me3
0.00
H4K20me1
0.10
H4K20me2
0.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TE2567832 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.2
0.00
Unmodified
0.00
H3K4me1
0.10
H3K4me2
0.10
H3K4me3
0.00
H3K9me1
0.00
H3K9me2
0.00
H3K9me3
0.00
H3K27me1
0.00
H3K27me2
100.00
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.00
H3K36me3
0.00
H4K20me1
0.10
H4K20me2
0.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11198 Lot #: TF2589871 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0045.4
0.00
Unmodified
0.00
H3K4me1
0.10
H3K4me2
0.10
H3K4me3
0.00
H3K9me1
0.00
H3K9me2
0.10
H3K9me3
0.00
H3K27me1
0.00
H3K27me2
100.00
H3K27me3
0.10
H3K36me1
0.00
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.00
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9733 Lot #: 8 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 409
E0332.1
0.10
Unmodified
0.10
H3K4me1
0.10
H3K4me2
0.10
H3K4me3
0.10
H3K9me1
0.10
H3K9me2
0.10
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
100.00
H3K27me3
0.20
H3K36me1
0.20
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: Active Motif Catalog #: 39055 Lot #: 31618020 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0683.1
0.10
Unmodified
0.10
H3K4me1
0.20
H3K4me2
0.20
H3K4me3
0.10
H3K9me1
0.20
H3K9me2
0.40
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
100.00
H3K27me3
0.20
H3K36me1
0.10
H3K36me2
0.20
H3K36me3
0.20
H4K20me1
0.20
H4K20me2
0.20
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: A15024 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 2
E0379.2
0.10
Unmodified
0.10
H3K4me1
0.10
H3K4me2
0.40
H3K4me3
0.10
H3K9me1
0.10
H3K9me2
1.50
H3K9me3
0.10
H3K27me1
0.40
H3K27me2
100.00
H3K27me3
0.10
H3K36me1
0.20
H3K36me2
0.30
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.40
H4K20me3
Vendor: Abcam Catalog #: ab6002 Lot #: GR3232152-1 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 653
E0478.1
0.30
Unmodified
0.30
H3K4me1
0.30
H3K4me2
0.30
H3K4me3
0.40
H3K9me1
0.30
H3K9me2
0.30
H3K9me3
0.30
H3K27me1
2.20
H3K27me2
100.00
H3K27me3
0.50
H3K36me1
0.50
H3K36me2
0.40
H3K36me3
0.50
H4K20me1
0.30
H4K20me2
0.50
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33081 Lot #: UG2810495 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0649.1
0.20
Unmodified
0.50
H3K4me1
0.50
H3K4me2
0.50
H3K4me3
0.20
H3K9me1
0.20
H3K9me2
1.90
H3K9me3
0.50
H3K27me1
0.40
H3K27me2
100.00
H3K27me3
0.30
H3K36me1
0.30
H3K36me2
0.90
H3K36me3
0.30
H4K20me1
0.50
H4K20me2
6.60
H4K20me3
Vendor: EpiCypher Catalog #: 13-0030 Lot #: 18303001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0777.1
0.30
Unmodified
0.60
H3K4me1
0.60
H3K4me2
1.70
H3K4me3
0.30
H3K9me1
0.50
H3K9me2
3.00
H3K9me3
0.60
H3K27me1
0.60
H3K27me2
100.00
H3K27me3
0.50
H3K36me1
0.50
H3K36me2
2.40
H3K36me3
0.30
H4K20me1
0.60
H4K20me2
6.60
H4K20me3
Vendor: EMD Millipore Catalog #: 07-449 Lot #: 3253330 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 1614
E0687.1
0.40
Unmodified
0.40
H3K4me1
0.30
H3K4me2
1.60
H3K4me3
0.20
H3K9me1
0.30
H3K9me2
11.20
H3K9me3
0.20
H3K27me1
0.60
H3K27me2
100.00
H3K27me3
0.30
H3K36me1
0.30
H3K36me2
0.40
H3K36me3
0.30
H4K20me1
0.30
H4K20me2
2.30
H4K20me3
Vendor: Diagenode Catalog #: C15200181 Lot #: 001-12 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 2
E0685.1
0.20
Unmodified
0.20
H3K4me1
0.20
H3K4me2
0.20
H3K4me3
0.20
H3K9me1
0.20
H3K9me2
0.50
H3K9me3
0.70
H3K27me1
32.80
H3K27me2
100.00
H3K27me3
0.20
H3K36me1
0.30
H3K36me2
0.50
H3K36me3
0.20
H4K20me1
0.20
H4K20me2
0.20
H4K20me3
Vendor: Active Motif Catalog #: 61018 Lot #: 17019020 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 1
E0684.1
5.80
Unmodified
6.40
H3K4me1
5.10
H3K4me2
3.90
H3K4me3
3.50
H3K9me1
3.60
H3K9me2
5.10
H3K9me3
4.20
H3K27me1
6.50
H3K27me2
100.00
H3K27me3
5.40
H3K36me1
4.90
H3K36me2
6.30
H3K36me3
4.00
H4K20me1
5.30
H4K20me2
4.20
H4K20me3
Vendor: RevMAb Catalog #: 31-1062-00 Lot #: Q-06-01079 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0037.1
0.36
Unmodified
0.51
H3K4me1
0.39
H3K4me2
2.72
H3K4me3
0.29
H3K9me1
0.44
H3K9me2
6.49
H3K9me3
0.37
H3K27me1
1.12
H3K27me2
100.00
H3K27me3
0.30
H3K36me1
0.56
H3K36me2
3.44
H3K36me3
0.38
H4K20me1
0.56
H4K20me2
13.70
H4K20me3
Vendor: Diagenode Catalog #: C15410195 Lot #: A0824D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 60
E0686.1
0.30
Unmodified
0.30
H3K4me1
0.90
H3K4me2
6.30
H3K4me3
0.20
H3K9me1
0.70
H3K9me2
20.00
H3K9me3
0.40
H3K27me1
4.80
H3K27me2
100.00
H3K27me3
0.60
H3K36me1
0.50
H3K36me2
2.00
H3K36me3
0.50
H4K20me1
0.80
H4K20me2
4.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33081 Lot #: VC2972315 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0649.2
0.60
Unmodified
0.80
H3K4me1
0.80
H3K4me2
8.90
H3K4me3
0.40
H3K9me1
1.20
H3K9me2
11.90
H3K9me3
1.40
H3K27me1
2.70
H3K27me2
100.00
H3K27me3
0.70
H3K36me1
0.70
H3K36me2
12.60
H3K36me3
0.70
H4K20me1
1.10
H4K20me2
64.10
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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