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H4K20me3

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 5737 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 5
E0528.1
11.03%
5.55
Unmodified
2.60
H3K4me1
5.70
H3K4me2
7.62
H3K4me3
7.45
H3K9me1
5.22
H3K9me2
1.34
H3K9me3
4.65
H3K27me1
4.94
H3K27me2
4.48
H3K27me3
3.25
H3K36me1
6.23
H3K36me2
6.78
H3K36me3
1.31
H4K20me1
9.33
H4K20me2
100.00
H4K20me3
Vendor: EpiGentek Catalog #: A-4048-025 Lot #: 8060412 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0497.1
7.89%
5.17
Unmodified
2.34
H3K4me1
6.19
H3K4me2
47.11
H3K4me3
6.43
H3K9me1
3.92
H3K9me2
0.95
H3K9me3
3.48
H3K27me1
4.57
H3K27me2
5.70
H3K27me3
2.53
H3K36me1
5.11
H3K36me2
8.64
H3K36me3
1.20
H4K20me1
6.93
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18074 Lot #: N/I Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0200.1
0.17%
18.65
Unmodified
18.45
H3K4me1
8.24
H3K4me2
10.69
H3K4me3
7.52
H3K9me1
11.74
H3K9me2
4.21
H3K9me3
11.73
H3K27me1
4.29
H3K27me2
9.30
H3K27me3
12.61
H3K36me1
10.29
H3K36me2
15.32
H3K36me3
9.54
H4K20me1
16.66
H4K20me2
100.00
H4K20me3
Vendor: Abcam Catalog #: ab177190 Lot #: GR220651-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0431.1
6.90%
8.71
Unmodified
4.26
H3K4me1
10.63
H3K4me2
51.59
H3K4me3
9.85
H3K9me1
5.85
H3K9me2
3.07
H3K9me3
8.44
H3K27me1
8.10
H3K27me2
28.38
H3K27me3
6.97
H3K36me1
9.51
H3K36me2
23.87
H3K36me3
2.39
H4K20me1
12.33
H4K20me2
100.00
H4K20me3
Vendor: Diagenode Catalog #: C15410057-10 Lot #: A295-0014P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 12
E0529.1
8.85%
11.90
Unmodified
5.50
H3K4me1
15.52
H3K4me2
67.70
H3K4me3
16.09
H3K9me1
9.57
H3K9me2
3.71
H3K9me3
7.25
H3K27me1
10.18
H3K27me2
28.45
H3K27me3
6.86
H3K36me1
11.07
H3K36me2
23.75
H3K36me3
2.25
H4K20me1
16.89
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701777 Lot #: RC227724 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0123.1
7.27%
11.37
Unmodified
17.52
H3K4me1
20.06
H3K4me2
50.73
H3K4me3
7.96
H3K9me1
11.98
H3K9me2
97.07
H3K9me3
22.85
H3K27me1
9.82
H3K27me2
24.48
H3K27me3
17.77
H3K36me1
11.73
H3K36me2
29.35
H3K36me3
13.21
H4K20me1
34.76
H4K20me2
100.00
H4K20me3
Vendor: ABclonal Catalog #: A2372 Lot #: 2200150202 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0370.3
4.58%
5.21
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
1.12
H4K20me1
7.14
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18074 Lot #: SF2392351A Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0200.2
0.58%
9.01
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
6.83
H4K20me1
12.59
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710809 Lot #: RC226287 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0605.1
0.17%
14.38
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
2.79
H4K20me1
16.69
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40089 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0197.1
2.87%
31.58
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
49.49
H4K20me1
43.09
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720086 Lot #: RA226273 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0191.1
0.85%
43.14
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
79.44
H4K20me1
64.62
H4K20me2
100.00
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me3
Vendor: EpiGentek Catalog #: A-4048-025 Lot #: 8060412 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0497.1
0.10
Unmodified
0.10
H3K4me1
0.20
H3K4me2
1.50
H3K4me3
0.20
H3K9me1
0.10
H3K9me2
0.60
H3K9me3
0.20
H3K27me1
0.20
H3K27me2
0.60
H3K27me3
0.20
H3K36me1
0.20
H3K36me2
0.20
H3K36me3
0.20
H4K20me1
0.20
H4K20me2
100.00
H4K20me3
Vendor: ABclonal Catalog #: A2372 Lot #: 2200150202 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0370.3
0.20
Unmodified
0.20
H3K4me1
0.20
H3K4me2
1.60
H3K4me3
0.20
H3K9me1
0.20
H3K9me2
0.50
H3K9me3
0.20
H3K27me1
0.20
H3K27me2
0.50
H3K27me3
0.20
H3K36me1
0.10
H3K36me2
0.20
H3K36me3
0.30
H4K20me1
0.20
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18074 Lot #: N/I Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0200.1
0.40
Unmodified
0.60
H3K4me1
0.40
H3K4me2
0.30
H3K4me3
0.40
H3K9me1
0.50
H3K9me2
0.50
H3K9me3
0.40
H3K27me1
0.30
H3K27me2
0.50
H3K27me3
0.40
H3K36me1
0.50
H3K36me2
0.40
H3K36me3
0.40
H4K20me1
3.50
H4K20me2
100.00
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 5737 Lot #: 5 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 5
E0528.1
0.60
Unmodified
0.80
H3K4me1
0.80
H3K4me2
0.80
H3K4me3
0.80
H3K9me1
0.90
H3K9me2
0.90
H3K9me3
0.70
H3K27me1
0.70
H3K27me2
1.00
H3K27me3
0.80
H3K36me1
0.80
H3K36me2
0.90
H3K36me3
0.90
H4K20me1
1.00
H4K20me2
100.00
H4K20me3
Vendor: Diagenode Catalog #: C15410057-10 Lot #: A295-0014P Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 12
E0529.1
0.25
Unmodified
0.29
H3K4me1
2.60
H3K4me2
3.17
H3K4me3
0.24
H3K9me1
0.35
H3K9me2
1.72
H3K9me3
0.30
H3K27me1
0.35
H3K27me2
4.61
H3K27me3
0.27
H3K36me1
0.33
H3K36me2
1.47
H3K36me3
0.25
H4K20me1
0.38
H4K20me2
100.00
H4K20me3
Vendor: Abcam Catalog #: ab177190 Lot #: GR220651-2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0431.1
0.80
Unmodified
0.60
H3K4me1
0.70
H3K4me2
1.70
H3K4me3
1.40
H3K9me1
0.70
H3K9me2
2.30
H3K9me3
1.00
H3K27me1
1.00
H3K27me2
4.10
H3K27me3
0.90
H3K36me1
1.10
H3K36me2
1.60
H3K36me3
1.30
H4K20me1
1.40
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701777 Lot #: RC227724 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0123.1
0.40
Unmodified
0.50
H3K4me1
0.90
H3K4me2
39.50
H3K4me3
0.30
H3K9me1
0.50
H3K9me2
2.30
H3K9me3
0.30
H3K27me1
0.30
H3K27me2
1.40
H3K27me3
0.30
H3K36me1
0.40
H3K36me2
0.30
H3K36me3
0.30
H4K20me1
2.10
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40089 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0197.1
0.70
Unmodified
0.80
H3K4me1
2.30
H3K4me2
18.30
H3K4me3
0.80
H3K9me1
1.60
H3K9me2
14.10
H3K9me3
0.80
H3K27me1
1.10
H3K27me2
15.30
H3K27me3
0.80
H3K36me1
1.90
H3K36me2
5.90
H3K36me3
1.00
H4K20me1
1.70
H4K20me2
100.00
H4K20me3
Vendor: Abcam Catalog #: ab9053 Lot #: GR3227061-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 161
E0430.1
1.30
Unmodified
1.70
H3K4me1
3.20
H3K4me2
11.00
H3K4me3
2.10
H3K9me1
2.70
H3K9me2
10.70
H3K9me3
2.00
H3K27me1
3.20
H3K27me2
12.00
H3K27me3
1.60
H3K36me1
2.20
H3K36me2
3.70
H3K36me3
2.30
H4K20me1
7.40
H4K20me2
100.00
H4K20me3
Vendor: EMD Millipore Catalog #: 07-463 Lot #: 2972040 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 90
E0442.1
24.40
Unmodified
28.70
H3K4me1
28.40
H3K4me2
29.20
H3K4me3
32.60
H3K9me1
29.00
H3K9me2
36.60
H3K9me3
23.90
H3K27me1
34.90
H3K27me2
45.00
H3K27me3
34.00
H3K36me1
33.00
H3K36me2
32.30
H3K36me3
35.00
H4K20me1
25.80
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710809 Lot #: RC226287 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0605.1
23.50
Unmodified
70.90
H3K4me1
75.10
H3K4me2
66.20
H3K4me3
21.50
H3K9me1
33.80
H3K9me2
76.60
H3K9me3
65.80
H3K27me1
31.00
H3K27me2
88.30
H3K27me3
27.20
H3K36me1
153.50
H3K36me2
28.30
H3K36me3
28.30
H4K20me1
31.50
H4K20me2
100.00
H4K20me3
Vendor: RevMAb Catalog #: 31-1096-00 Lot #: Q-601089 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0125.1
78.63
Unmodified
111.97
H3K4me1
111.97
H3K4me2
82.05
H3K4me3
80.34
H3K9me1
107.69
H3K9me2
105.13
H3K9me3
63.25
H3K27me1
65.81
H3K27me2
92.31
H3K27me3
64.96
H3K36me1
111.11
H3K36me2
92.31
H3K36me3
82.05
H4K20me1
70.09
H4K20me2
100.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720086 Lot #: RA226273 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0191.1
70.00
Unmodified
88.10
H3K4me1
77.70
H3K4me2
85.10
H3K4me3
86.20
H3K9me1
111.10
H3K9me2
121.40
H3K9me3
86.10
H3K27me1
83.70
H3K27me2
91.80
H3K27me3
107.70
H3K36me1
127.50
H3K36me2
74.50
H3K36me3
84.50
H4K20me1
83.80
H4K20me2
100.00
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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