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H4K20me2

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me2
Vendor: Thermo Fisher Scientific Catalog #: 720085 Lot #: RC226269 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0121.1
3.27%
19.20
Unmodified
10.10
H3K4me1
44.63
H3K4me2
18.94
H3K4me3
20.33
H3K9me1
19.82
H3K9me2
4.26
H3K9me3
15.52
H3K27me1
20.38
H3K27me2
11.81
H3K27me3
8.71
H3K36me1
20.64
H3K36me2
16.23
H3K36me3
5.99
H4K20me1
100.00
H4K20me2
20.38
H4K20me3
Vendor: Diagenode Catalog #: C15200205 Lot #: 41582 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 1
E0689.1
0.23%
23.79
Unmodified
19.96
H3K4me1
37.02
H3K4me2
30.41
H3K4me3
24.75
H3K9me1
26.26
H3K9me2
34.67
H3K9me3
33.04
H3K27me1
27.94
H3K27me2
28.56
H3K27me3
29.03
H3K36me1
37.49
H3K36me2
26.67
H3K36me3
23.65
H4K20me1
100.00
H4K20me2
33.67
H4K20me3
Vendor: Diagenode Catalog #: C15200220 Lot #: 001-11 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0372.1
0.35%
23.70
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
50.94
H4K20me1
100.00
H4K20me2
23.84
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-31511 Lot #: UH2819164A Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0665.1
1.04%
45.24
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
16.43
H4K20me1
100.00
H4K20me2
41.78
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-31510 Lot #: UH2819163 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0664.1
4.67%
59.71
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
10.07
H4K20me1
100.00
H4K20me2
54.66
H4K20me3
Vendor: ABclonal Catalog #: A2371 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0122.1
0.03%
302.67
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
23.62
H4K20me1
100.00
H4K20me2
32.87
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me2
Vendor: Thermo Fisher Scientific Catalog #: MA5-31511 Lot #: UH2819164A Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0665.1
0.10
Unmodified
0.40
H3K4me1
0.40
H3K4me2
0.30
H3K4me3
0.20
H3K9me1
0.30
H3K9me2
0.30
H3K9me3
0.20
H3K27me1
0.40
H3K27me2
0.30
H3K27me3
0.20
H3K36me1
0.10
H3K36me2
0.30
H3K36me3
1.20
H4K20me1
100.00
H4K20me2
0.60
H4K20me3
Vendor: Diagenode Catalog #: C15200205 Lot #: 41582 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 1
E0689.1
0.50
Unmodified
1.00
H3K4me1
0.40
H3K4me2
0.40
H3K4me3
0.30
H3K9me1
0.40
H3K9me2
0.40
H3K9me3
0.20
H3K27me1
0.30
H3K27me2
0.30
H3K27me3
0.30
H3K36me1
0.90
H3K36me2
0.50
H3K36me3
1.90
H4K20me1
100.00
H4K20me2
0.70
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-31510 Lot #: UH2819163 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0664.1
0.20
Unmodified
0.40
H3K4me1
0.40
H3K4me2
0.50
H3K4me3
0.10
H3K9me1
0.50
H3K9me2
0.30
H3K9me3
0.30
H3K27me1
0.30
H3K27me2
0.30
H3K27me3
0.20
H3K36me1
0.20
H3K36me2
0.50
H3K36me3
2.90
H4K20me1
100.00
H4K20me2
2.70
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720085 Lot #: RC226269 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0121.1
4.10
Unmodified
5.00
H3K4me1
5.40
H3K4me2
4.60
H3K4me3
4.00
H3K9me1
6.70
H3K9me2
5.80
H3K9me3
4.30
H3K27me1
5.60
H3K27me2
4.90
H3K27me3
4.00
H3K36me1
6.80
H3K36me2
3.60
H3K36me3
4.70
H4K20me1
100.00
H4K20me2
6.10
H4K20me3
Vendor: Abcam Catalog #: ab9052 Lot #: GR325279-4 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 39
E0428.1
44.40
Unmodified
43.60
H3K4me1
53.90
H3K4me2
36.90
H3K4me3
51.00
H3K9me1
66.40
H3K9me2
53.00
H3K9me3
58.30
H3K27me1
60.10
H3K27me2
54.50
H3K27me3
35.90
H3K36me1
54.80
H3K36me2
40.30
H3K36me3
63.40
H4K20me1
100.00
H4K20me2
50.70
H4K20me3
Vendor: EpiGentek Catalog #: A-4047-025 Lot #: 8060411 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0483.1
85.60
Unmodified
83.20
H3K4me1
84.80
H3K4me2
91.20
H3K4me3
102.40
H3K9me1
90.40
H3K9me2
84.80
H3K9me3
84.00
H3K27me1
96.40
H3K27me2
108.00
H3K27me3
83.20
H3K36me1
113.60
H3K36me2
111.20
H3K36me3
169.60
H4K20me1
100.00
H4K20me2
111.60
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9759 Lot #: 1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 9
E0527.1
78.50
Unmodified
89.60
H3K4me1
85.90
H3K4me2
85.90
H3K4me3
109.80
H3K9me1
125.80
H3K9me2
116.60
H3K9me3
83.40
H3K27me1
83.40
H3K27me2
93.30
H3K27me3
109.80
H3K36me1
121.50
H3K36me2
160.10
H3K36me3
123.30
H4K20me1
100.00
H4K20me2
96.90
H4K20me3
Vendor: Active Motif Catalog #: 39540 Lot #: 8308002 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 1
E0457.1
111.00
Unmodified
102.00
H3K4me1
109.00
H3K4me2
121.00
H3K4me3
102.00
H3K9me1
122.00
H3K9me2
97.00
H3K9me3
120.00
H3K27me1
89.00
H3K27me2
100.00
H3K27me3
90.00
H3K36me1
118.00
H3K36me2
115.00
H3K36me3
100.00
H4K20me1
100.00
H4K20me2
107.00
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 78781 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0526.1
62.10
Unmodified
75.40
H3K4me1
79.10
H3K4me2
81.40
H3K4me3
73.20
H3K9me1
389.80
H3K9me2
98.40
H3K9me3
73.80
H3K27me1
128.30
H3K27me2
78.70
H3K27me3
79.70
H3K36me1
124.40
H3K36me2
79.10
H3K36me3
104.90
H4K20me1
100.00
H4K20me2
94.70
H4K20me3
Vendor: Abcam Catalog #: ab51878 Lot #: GR27019-13 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0429.1
118.30
Unmodified
130.30
H3K4me1
91.90
H3K4me2
125.00
H3K4me3
117.60
H3K9me1
123.90
H3K9me2
105.60
H3K9me3
108.50
H3K27me1
109.90
H3K27me2
159.90
H3K27me3
130.30
H3K36me1
125.70
H3K36me2
128.90
H3K36me3
99.30
H4K20me1
100.00
H4K20me2
98.60
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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