Skip to Main Content

H4K20me1

Please Make a Selection

SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me1
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: TF2586184 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0117.3
6.34%
5.73
Unmodified
3.47
H3K4me1
5.61
H3K4me2
6.64
H3K4me3
6.28
H3K9me1
3.73
H3K9me2
1.01
H3K9me3
4.46
H3K27me1
4.90
H3K27me2
3.43
H3K27me3
1.34
H3K36me1
5.71
H3K36me2
4.91
H3K36me3
100.00
H4K20me1
6.88
H4K20me2
6.37
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: TF2589962 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0117.2
9.32%
6.14
Unmodified
2.49
H3K4me1
5.29
H3K4me2
6.47
H3K4me3
6.48
H3K9me1
3.96
H3K9me2
0.92
H3K9me3
5.05
H3K27me1
4.68
H3K27me2
3.72
H3K27me3
1.85
H3K36me1
5.89
H3K36me2
4.93
H3K36me3
100.00
H4K20me1
7.24
H4K20me2
6.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: TD2544432 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0117.1
1.93%
2.98
Unmodified
5.72
H3K4me1
2.42
H3K4me2
0.63
H3K4me3
1.78
H3K9me1
3.08
H3K9me2
5.72
H3K9me3
4.77
H3K27me1
0.87
H3K27me2
1.48
H3K27me3
3.26
H3K36me1
2.03
H3K36me2
3.74
H3K36me3
100.00
H4K20me1
2.09
H4K20me2
1.36
H4K20me3
Vendor: EMD Millipore Catalog #: 07-1570 Lot #: 2941503 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 5
E0441.1
12.34%
7.38
Unmodified
4.31
H3K4me1
8.61
H3K4me2
9.37
H3K4me3
9.44
H3K9me1
5.36
H3K9me2
0.00
H3K9me3
6.86
H3K27me1
5.89
H3K27me2
5.00
H3K27me3
23.01
H3K36me1
7.51
H3K36me2
7.40
H3K36me3
100.00
H4K20me1
8.71
H4K20me2
6.61
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: N/I Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0117.4
0.27%
21.40
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
100.00
H4K20me1
16.82
H4K20me2
10.20
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1023 Lot #: A254001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0146.1
0.22%
17.68
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
100.00
H4K20me1
21.69
H4K20me2
23.44
H4K20me3
Vendor: EpiGentek Catalog #: A-4046-025 Lot #: 8060410 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0484.1
0.05%
27.94
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
100.00
H4K20me1
34.20
H4K20me2
35.39
H4K20me3
Vendor: Abclonal Catalog #: A2370 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0119.1
0.26%
31.94
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
100.00
H4K20me1
44.75
H4K20me2
30.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720084 Lot #: RA226272 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0184.1
0.10%
29.70
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
100.00
H4K20me1
57.00
H4K20me2
25.05
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H4K20me1
Vendor: Abcam Catalog #: ab9051 Lot #: GR3284863-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 102
E0426.2
0.20
Unmodified
0.40
H3K4me1
0.20
H3K4me2
0.20
H3K4me3
0.20
H3K9me1
0.20
H3K9me2
0.20
H3K9me3
0.30
H3K27me1
0.20
H3K27me2
0.30
H3K27me3
0.20
H3K36me1
0.20
H3K36me2
0.20
H3K36me3
100.00
H4K20me1
0.20
H4K20me2
0.20
H4K20me3
Vendor: EMD Millipore Catalog #: 07-1570 Lot #: 2941503 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 5
E0441.1
0.30
Unmodified
0.30
H3K4me1
0.30
H3K4me2
0.50
H3K4me3
0.40
H3K9me1
0.90
H3K9me2
0.30
H3K9me3
0.30
H3K27me1
0.30
H3K27me2
0.40
H3K27me3
0.40
H3K36me1
0.40
H3K36me2
0.30
H3K36me3
100.00
H4K20me1
0.40
H4K20me2
0.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: TF2589962 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0117.2
0.40
Unmodified
0.70
H3K4me1
0.50
H3K4me2
0.40
H3K4me3
0.50
H3K9me1
0.30
H3K9me2
0.50
H3K9me3
0.60
H3K27me1
0.60
H3K27me2
0.70
H3K27me3
0.60
H3K36me1
0.50
H3K36me2
0.60
H3K36me3
100.00
H4K20me1
1.20
H4K20me2
2.20
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: TF2586184 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0117.3
0.50
Unmodified
0.70
H3K4me1
0.60
H3K4me2
0.40
H3K4me3
0.50
H3K9me1
0.30
H3K9me2
0.50
H3K9me3
0.70
H3K27me1
0.60
H3K27me2
0.80
H3K27me3
0.60
H3K36me1
0.50
H3K36me2
0.70
H3K36me3
100.00
H4K20me1
1.50
H4K20me2
2.70
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-18067 Lot #: N/I Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0117.4
1.40
Unmodified
1.80
H3K4me1
1.60
H3K4me2
1.20
H3K4me3
1.30
H3K9me1
0.90
H3K9me2
1.50
H3K9me3
1.80
H3K27me1
1.50
H3K27me2
1.90
H3K27me3
1.70
H3K36me1
1.40
H3K36me2
1.70
H3K36me3
100.00
H4K20me1
2.70
H4K20me2
2.90
H4K20me3
Vendor: EpiGentek Catalog #: A-4046-025 Lot #: 8060410 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0484.1
1.40
Unmodified
1.90
H3K4me1
1.30
H3K4me2
1.80
H3K4me3
2.00
H3K9me1
1.40
H3K9me2
1.60
H3K9me3
1.70
H3K27me1
1.50
H3K27me2
1.80
H3K27me3
2.40
H3K36me1
2.00
H3K36me2
1.80
H3K36me3
100.00
H4K20me1
1.60
H4K20me2
1.40
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1023 Lot #: A254001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0146.1
2.00
Unmodified
5.20
H3K4me1
3.00
H3K4me2
2.60
H3K4me3
8.20
H3K9me1
5.70
H3K9me2
3.00
H3K9me3
8.20
H3K27me1
3.30
H3K27me2
2.20
H3K27me3
2.60
H3K36me1
5.50
H3K36me2
1.40
H3K36me3
100.00
H4K20me1
8.20
H4K20me2
3.10
H4K20me3
Vendor: Diagenode Catalog #: C15410034 Lot #: A255-0010D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0525.1
1.28
Unmodified
1.80
H3K4me1
2.14
H3K4me2
2.65
H3K4me3
2.00
H3K9me1
11.22
H3K9me2
2.09
H3K9me3
1.93
H3K27me1
2.03
H3K27me2
2.32
H3K27me3
1.83
H3K36me1
2.06
H3K36me2
1.53
H3K36me3
100.00
H4K20me1
6.12
H4K20me2
8.95
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9724 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0283.1
6.00
Unmodified
7.90
H3K4me1
12.70
H3K4me2
14.00
H3K4me3
11.00
H3K9me1
10.90
H3K9me2
9.40
H3K9me3
12.70
H3K27me1
9.70
H3K27me2
11.50
H3K27me3
11.10
H3K36me1
14.60
H3K36me2
10.10
H3K36me3
100.00
H4K20me1
8.00
H4K20me2
11.80
H4K20me3
Vendor: Diagenode Catalog #: C15200147 Lot #: 1 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 3
E0499.1
54.90
Unmodified
82.60
H3K4me1
56.20
H3K4me2
63.60
H3K4me3
47.40
H3K9me1
42.60
H3K9me2
66.90
H3K9me3
82.30
H3K27me1
51.80
H3K27me2
79.70
H3K27me3
55.60
H3K36me1
70.30
H3K36me2
47.90
H3K36me3
100.00
H4K20me1
59.20
H4K20me2
20.50
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720084 Lot #: RA226272 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0184.1
66.50
Unmodified
99.80
H3K4me1
69.40
H3K4me2
80.90
H3K4me3
74.80
H3K9me1
111.30
H3K9me2
99.10
H3K9me3
72.40
H3K27me1
73.30
H3K27me2
84.60
H3K27me3
78.20
H3K36me1
108.10
H3K36me2
61.50
H3K36me3
100.00
H4K20me1
85.90
H4K20me2
88.10
H4K20me3
Vendor: Abcam Catalog #: ab16974 Lot #: 143271 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0427.2
84.20
Unmodified
113.20
H3K4me1
56.10
H3K4me2
68.40
H3K4me3
98.20
H3K9me1
112.30
H3K9me2
94.70
H3K9me3
71.90
H3K27me1
73.70
H3K27me2
84.20
H3K27me3
93.90
H3K36me1
84.20
H3K36me2
86.00
H3K36me3
100.00
H4K20me1
91.20
H4K20me2
77.20
H4K20me3
Vendor: Abcam Catalog #: ab177188 Lot #: GR188305-4 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0498.2
78.70
Unmodified
86.00
H3K4me1
69.50
H3K4me2
166.50
H3K4me3
101.20
H3K9me1
78.00
H3K9me2
92.10
H3K9me3
72.00
H3K27me1
95.10
H3K27me2
76.20
H3K27me3
90.20
H3K36me1
82.90
H3K36me2
77.40
H3K36me3
100.00
H4K20me1
85.40
H4K20me2
73.20
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
Back to Homepage