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H3K9me1

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K9me1
Vendor: Thermo Fisher Scientific Catalog #: MA5-33385 Lot #: UI2850858 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0870.1
10.78%
1.60
Unmodified
1.82
H3K4me1
2.27
H3K4me2
0.95
H3K4me3
100.00
H3K9me1
1.75
H3K9me2
4.12
H3K9me3
5.35
H3K27me1
1.85
H3K27me2
1.72
H3K27me3
2.75
H3K36me1
2.69
H3K36me2
2.59
H3K36me3
1.67
H4K20me1
2.02
H4K20me2
1.85
H4K20me3
Vendor: RevMAb Catalog #: 31-1064-00 Lot #: R-02-01480 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0258.2
10.78%
1.60
Unmodified
1.82
H3K4me1
2.27
H3K4me2
0.95
H3K4me3
100.00
H3K9me1
1.75
H3K9me2
4.12
H3K9me3
5.35
H3K27me1
1.85
H3K27me2
1.72
H3K27me3
2.75
H3K36me1
2.69
H3K36me2
2.59
H3K36me3
1.67
H4K20me1
2.02
H4K20me2
1.85
H4K20me3
Vendor: EpiCypher Catalog #: 13-0029 Lot #: 18344001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0776.1
46.81%
13.91
Unmodified
10.68
H3K4me1
13.95
H3K4me2
12.66
H3K4me3
100.00
H3K9me1
9.56
H3K9me2
8.33
H3K9me3
15.38
H3K27me1
11.63
H3K27me2
14.70
H3K27me3
15.57
H3K36me1
18.06
H3K36me2
16.86
H3K36me3
10.40
H4K20me1
15.58
H4K20me2
10.87
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720091 Lot #: QL226265 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0186.1
4.22%
6.95
Unmodified
11.31
H3K4me1
7.81
H3K4me2
9.01
H3K4me3
100.00
H3K9me1
6.99
H3K9me2
7.94
H3K9me3
37.29
H3K27me1
10.35
H3K27me2
6.94
H3K27me3
11.40
H3K36me1
9.92
H3K36me2
11.17
H3K36me3
9.31
H4K20me1
8.67
H4K20me2
6.87
H4K20me3
Vendor: EpiCypher Catalog #: 13-0014 Lot #: 14247001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0160.1
7.46%
4.89
Unmodified
20.39
H3K4me1
7.25
H3K4me2
19.74
H3K4me3
100.00
H3K9me1
6.45
H3K9me2
10.76
H3K9me3
16.19
H3K27me1
3.53
H3K27me2
4.99
H3K27me3
21.79
H3K36me1
9.12
H3K36me2
19.73
H3K36me3
19.95
H4K20me1
16.36
H4K20me2
3.81
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710814 Lot #: RJ241996 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0236.1
12.36%
12.18
Unmodified
45.03
H3K4me1
15.93
H3K4me2
20.65
H3K4me3
100.00
H3K9me1
14.57
H3K9me2
19.24
H3K9me3
21.20
H3K27me1
11.74
H3K27me2
13.49
H3K27me3
26.23
H3K36me1
15.70
H3K36me2
22.38
H3K36me3
18.70
H4K20me1
17.67
H4K20me2
8.89
H4K20me3
Vendor: Abclonal Catalog #: A2358 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 2
E0083.1
5.67%
9.73
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
100.00
H3K9me1
12.03
H3K9me2
6.60
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EpiGentek Catalog #: A-4034 Lot #: 705669 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0084.1
4.79%
9.26
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
100.00
H3K9me1
13.43
H3K9me2
6.75
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1006 Lot #: A89001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0203.1
0.94%
17.81
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
100.00
H3K9me1
20.28
H3K9me2
18.17
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K9me1
Vendor: RevMAb Catalog #: 31-1064-00 Lot #: R-02-01480 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0258.2
0.10
Unmodified
0.20
H3K4me1
0.10
H3K4me2
0.10
H3K4me3
100.00
H3K9me1
0.10
H3K9me2
0.10
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
0.20
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1006 Lot #: A89001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0203.1
0.70
Unmodified
11.40
H3K4me1
0.70
H3K4me2
0.70
H3K4me3
100.00
H3K9me1
0.70
H3K9me2
0.80
H3K9me3
0.90
H3K27me1
0.70
H3K27me2
0.70
H3K27me3
0.70
H3K36me1
0.80
H3K36me2
0.60
H3K36me3
0.70
H4K20me1
0.60
H4K20me2
0.70
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710814 Lot #: RJ241996 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0236.1
1.40
Unmodified
13.60
H3K4me1
5.60
H3K4me2
6.20
H3K4me3
100.00
H3K9me1
2.20
H3K9me2
3.40
H3K9me3
5.30
H3K27me1
5.30
H3K27me2
5.30
H3K27me3
3.50
H3K36me1
3.50
H3K36me2
5.00
H3K36me3
2.20
H4K20me1
2.70
H4K20me2
2.80
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720091 Lot #: QL226265 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0186.1
3.40
Unmodified
4.50
H3K4me1
5.80
H3K4me2
4.10
H3K4me3
100.00
H3K9me1
12.00
H3K9me2
5.10
H3K9me3
11.00
H3K27me1
4.60
H3K27me2
5.80
H3K27me3
6.70
H3K36me1
5.80
H3K36me2
3.50
H3K36me3
4.70
H4K20me1
10.00
H4K20me2
4.60
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701782 Lot #: 2024880 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0644.1
21.70
Unmodified
23.90
H3K4me1
32.40
H3K4me2
20.30
H3K4me3
100.00
H3K9me1
20.50
H3K9me2
23.90
H3K9me3
25.40
H3K27me1
26.10
H3K27me2
24.60
H3K27me3
26.80
H3K36me1
28.60
H3K36me2
27.20
H3K36me3
26.30
H4K20me1
31.30
H4K20me2
26.10
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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