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H3K4me3

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K4me3
Vendor: EpiCypher Catalog #: 13-0028 Lot #: 18303001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0775.1
6.95%
1.13
Unmodified
0.31
H3K4me1
1.31
H3K4me2
100.00
H3K4me3
0.96
H3K9me1
0.40
H3K9me2
0.39
H3K9me3
0.41
H3K27me1
0.79
H3K27me2
0.52
H3K27me3
0.12
H3K36me1
0.85
H3K36me2
3.74
H3K36me3
0.09
H4K20me1
0.68
H4K20me2
0.99
H4K20me3
Vendor: EpiCypher Catalog #: 13-0041 Lot #: 20010001-38 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0785.1
63.40%
1.51
Unmodified
0.40
H3K4me1
0.14
H3K4me2
100.00
H3K4me3
1.15
H3K9me1
0.71
H3K9me2
1.37
H3K9me3
0.75
H3K27me1
0.96
H3K27me2
0.88
H3K27me3
0.27
H3K36me1
0.95
H3K36me2
4.45
H3K36me3
0.18
H4K20me1
0.87
H4K20me2
1.82
H4K20me3
Vendor: EpiCypher Catalog #: 13-0041 Lot #: 20083002-42 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0785.2
70.85%
0.66
Unmodified
0.39
H3K4me1
3.79
H3K4me2
100.00
H3K4me3
0.54
H3K9me1
0.34
H3K9me2
1.92
H3K9me3
0.42
H3K27me1
0.43
H3K27me2
0.93
H3K27me3
0.20
H3K36me1
0.52
H3K36me2
6.16
H3K36me3
0.14
H4K20me1
0.52
H4K20me2
0.75
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 711958 Lot #: 2203285 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0609.3
70.85%
0.66
Unmodified
0.39
H3K4me1
3.79
H3K4me2
100.00
H3K4me3
0.54
H3K9me1
0.34
H3K9me2
1.92
H3K9me3
0.42
H3K27me1
0.43
H3K27me2
0.93
H3K27me3
0.20
H3K36me1
0.52
H3K36me2
6.16
H3K36me3
0.14
H4K20me1
0.52
H4K20me2
0.75
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 711958 Lot #: BLR05042019-02 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0609.2
61.75%
1.11
Unmodified
0.58
H3K4me1
4.42
H3K4me2
100.00
H3K4me3
0.73
H3K9me1
0.53
H3K9me2
1.94
H3K9me3
0.61
H3K27me1
0.65
H3K27me2
1.01
H3K27me3
0.19
H3K36me1
0.78
H3K36me2
6.52
H3K36me3
0.16
H4K20me1
0.76
H4K20me2
1.07
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: UL2896063 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.2
18.37%
0.99
Unmodified
0.38
H3K4me1
3.42
H3K4me2
100.00
H3K4me3
1.02
H3K9me1
2.69
H3K9me2
0.80
H3K9me3
0.70
H3K27me1
0.68
H3K27me2
2.44
H3K27me3
0.47
H3K36me1
0.66
H3K36me2
11.55
H3K36me3
0.12
H4K20me1
2.64
H4K20me2
1.02
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: UL2906023 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.1
18.37%
0.99
Unmodified
0.38
H3K4me1
3.42
H3K4me2
100.00
H3K4me3
1.02
H3K9me1
2.69
H3K9me2
0.80
H3K9me3
0.70
H3K27me1
0.68
H3K27me2
2.44
H3K27me3
0.47
H3K36me1
0.66
H3K36me2
11.55
H3K36me3
0.12
H4K20me1
2.64
H4K20me2
1.02
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: VB2941981 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.3
18.37%
0.99
Unmodified
0.38
H3K4me1
3.42
H3K4me2
100.00
H3K4me3
1.02
H3K9me1
2.69
H3K9me2
0.80
H3K9me3
0.70
H3K27me1
0.68
H3K27me2
2.44
H3K27me3
0.47
H3K36me1
0.66
H3K36me2
11.55
H3K36me3
0.12
H4K20me1
2.64
H4K20me2
1.02
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: VB2942393 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.4
18.37%
0.99
Unmodified
0.38
H3K4me1
3.42
H3K4me2
100.00
H3K4me3
1.02
H3K9me1
2.69
H3K9me2
0.80
H3K9me3
0.70
H3K27me1
0.68
H3K27me2
2.44
H3K27me3
0.47
H3K36me1
0.66
H3K36me2
11.55
H3K36me3
0.12
H4K20me1
2.64
H4K20me2
1.02
H4K20me3
Vendor: RevMAb Catalog #: 31-1226-00 Lot #: S-07-02385 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0231.3
18.37%
0.99
Unmodified
0.38
H3K4me1
3.42
H3K4me2
100.00
H3K4me3
1.02
H3K9me1
2.69
H3K9me2
0.80
H3K9me3
0.70
H3K27me1
0.68
H3K27me2
2.44
H3K27me3
0.47
H3K36me1
0.66
H3K36me2
11.55
H3K36me3
0.12
H4K20me1
2.64
H4K20me2
1.02
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: UI2850856 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.5
8.79%
1.39
Unmodified
5.45
H3K4me1
3.01
H3K4me2
100.00
H3K4me3
1.52
H3K9me1
1.50
H3K9me2
1.19
H3K9me3
1.93
H3K27me1
1.50
H3K27me2
4.66
H3K27me3
1.13
H3K36me1
1.67
H3K36me2
4.10
H3K36me3
1.01
H4K20me1
2.81
H4K20me2
1.06
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-33382 Lot #: UK2886824 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0844.6
8.79%
1.39
Unmodified
5.45
H3K4me1
3.01
H3K4me2
100.00
H3K4me3
1.52
H3K9me1
1.50
H3K9me2
1.19
H3K9me3
1.93
H3K27me1
1.50
H3K27me2
4.66
H3K27me3
1.13
H3K36me1
1.67
H3K36me2
4.10
H3K36me3
1.01
H4K20me1
2.81
H4K20me2
1.06
H4K20me3
Vendor: RevMAb Catalog #: 31-1226-00 Lot #: R-02-01481 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0231.1
8.79%
1.39
Unmodified
5.45
H3K4me1
3.01
H3K4me2
100.00
H3K4me3
1.52
H3K9me1
1.50
H3K9me2
1.19
H3K9me3
1.93
H3K27me1
1.50
H3K27me2
4.66
H3K27me3
1.13
H3K36me1
1.67
H3K36me2
4.10
H3K36me3
1.01
H4K20me1
2.81
H4K20me2
1.06
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-27029 Lot #: SG2419844A Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 2
E0193.1
53.07%
2.57
Unmodified
2.58
H3K4me1
9.49
H3K4me2
100.00
H3K4me3
0.94
H3K9me1
0.73
H3K9me2
5.29
H3K9me3
1.56
H3K27me1
0.81
H3K27me2
2.83
H3K27me3
2.92
H3K36me1
0.83
H3K36me2
10.20
H3K36me3
2.28
H4K20me1
2.03
H4K20me2
0.72
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 703954 Lot #: BLR19122019-03 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0848.1
74.77%
3.11
Unmodified
1.19
H3K4me1
12.90
H3K4me2
100.00
H3K4me3
2.21
H3K9me1
1.77
H3K9me2
5.51
H3K9me3
1.60
H3K27me1
2.21
H3K27me2
3.59
H3K27me3
0.87
H3K36me1
1.92
H3K36me2
7.34
H3K36me3
0.67
H4K20me1
1.59
H4K20me2
5.42
H4K20me3
Vendor: EpiCypher Catalog #: 13-0004 Lot #: 13171001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0149.1
0.84%
4.84
Unmodified
8.63
H3K4me1
24.11
H3K4me2
100.00
H3K4me3
2.19
H3K9me1
11.36
H3K9me2
11.12
H3K9me3
4.08
H3K27me1
2.68
H3K27me2
2.15
H3K27me3
1.76
H3K36me1
2.04
H3K36me2
4.49
H3K36me3
1.16
H4K20me1
3.08
H4K20me2
2.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40086 Lot #: SF2392265B Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0360.1
15.84%
0.27
Unmodified
0.29
H3K4me1
1.86
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40086 Lot #: RL2301825 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0360.3*
16.82%
0.34
Unmodified
0.05
H3K4me1
3.24
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40086 Lot #: SC2349865 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0360.2
9.80%
0.60
Unmodified
0.21
H3K4me1
2.43
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2357 Lot #: 46698 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 6
E0399.1*
13.05%
0.92
Unmodified
0.44
H3K4me1
8.92
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab12209 Lot #: GR275790-1 Clonality: Monoclonal Species: Mouse Pass/Fail: Limited Data # Citations: 35
E0627.1*
5.20%
2.32
Unmodified
1.36
H3K4me1
10.04
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410003 Lot #: A1052D Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 201
E0356.1
45.71%
0.16
Unmodified
0.84
H3K4me1
13.21
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2357 Lot #: 46699 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 6
E0399.2*
42.95%
0.38
Unmodified
1.46
H3K4me1
14.24
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EpiGentek Catalog #: A-4033-050 Lot #: 606361 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 2
E0341.1*
47.87%
0.65
Unmodified
2.12
H3K4me1
17.35
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 703849 Lot #: BLR20062019-02 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0663.1
1.16%
0.53
Unmodified
0.43
H3K4me1
10.48
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-27029 Lot #: 43727 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0193.3
85.22%
2.28
Unmodified
1.71
H3K4me1
27.49
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Koide Lab Catalog #: 304M3B Lot #: 040416AG Clonality: Species: Pass/Fail: Fail # Citations: 0
E0839.1*
36.16%
0.88
Unmodified
4.45
H3K4me1
26.29
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-27029 Lot #: UI283051 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0193.2
82.99%
2.06
Unmodified
1.83
H3K4me1
28.58
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410003 Lot #: A1052D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 201
E0356.1*
40.28%
0.95
Unmodified
1.69
H3K4me1
36.81
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EMD Millipore Catalog #: 05-745R Lot #: 2813867 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 25
E0358.1*
55.18%
3.71
Unmodified
1.40
H3K4me1
34.38
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15200152 Lot #: 001-11 Clonality: Polyclonal Species: Mouse Pass/Fail: Fail # Citations: 4
E0351.1*
1.41%
5.61
Unmodified
9.31
H3K4me1
24.56
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Epicypher Catalog #: 13-0004 Lot #: 13171001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0149.1*
1.14%
8.49
Unmodified
2.49
H3K4me1
30.18
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Rockland Catalog #: 600-401-I59 Lot #: 32388 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0510.1
47.52%
4.10
Unmodified
6.31
H3K4me1
31.25
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9727 Lot #: 2 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 98
E0337.1
12.09%
0.37
Unmodified
1.44
H3K4me1
43.14
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Active Motif Catalog #: 61379 Lot #: 24615006 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 11
E0840.1*
0.43%
28.71
Unmodified
2.05
H3K4me1
17.41
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: RevMAb Catalog #: 31-1039-00 Lot #: P-09-00676 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0353.1*
41.93%
10.69
Unmodified
4.68
H3K4me1
35.09
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9727 Lot #: 2 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 98
E0337.1*
31.03%
0.40
Unmodified
1.41
H3K4me1
51.91
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410003 Lot #: A5051-001P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 201
E0356.2*
35.07%
0.41
Unmodified
2.77
H3K4me1
51.13
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Active Motif Catalog #: 39159 Lot #: 12613005 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 243
E0345.1*
5.36%
21.66
Unmodified
3.88
H3K4me1
29.16
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab8580 Lot #: GR190229-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1497
E0253.1*
63.44%
1.61
Unmodified
7.15
H3K4me1
58.18
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9751 Lot #: 9 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 227
E0841.1*
58.62%
1.54
Unmodified
7.56
H3K4me1
61.48
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EMD Millipore Catalog #: 07-473 Lot #: 2839113 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 769
E0339.1*
54.16%
0.68
Unmodified
3.31
H3K4me1
75.46
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab8580 Lot #: GR273043-4 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1497
E0253.2*
59.35%
1.49
Unmodified
10.09
H3K4me1
70.65
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab8580 Lot #: GR190229-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1497
E0253.1
67.83%
10.60
Unmodified
21.00
H3K4me1
55.62
H3K4me2
100.00
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K4me3
Vendor: Thermo Fisher Scientific Catalog #: 703849 Lot #: BLR20062019-02 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0663.1
0.10
Unmodified
0.50
H3K4me1
2.20
H3K4me2
100.00
H3K4me3
0.10
H3K9me1
0.10
H3K9me2
0.50
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
0.20
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-40086 Lot #: SF2392265B Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0360.1
0.10
Unmodified
0.30
H3K4me1
3.40
H3K4me2
100.00
H3K4me3
0.10
H3K9me1
0.10
H3K9me2
0.20
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
0.10
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: RevMAb Catalog #: 31-1226-00 Lot #: S-07-02385 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0231.3
0.30
Unmodified
0.40
H3K4me1
0.50
H3K4me2
100.00
H3K4me3
0.30
H3K9me1
0.50
H3K9me2
0.30
H3K9me3
0.40
H3K27me1
0.40
H3K27me2
0.40
H3K27me3
0.40
H3K36me1
0.40
H3K36me2
0.40
H3K36me3
0.40
H4K20me1
0.30
H4K20me2
0.30
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-27029 Lot #: SG2419844A Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 2
E0193.1
0.20
Unmodified
0.50
H3K4me1
8.40
H3K4me2
100.00
H3K4me3
0.20
H3K9me1
0.30
H3K9me2
1.00
H3K9me3
0.20
H3K27me1
0.20
H3K27me2
0.60
H3K27me3
0.20
H3K36me1
0.30
H3K36me2
0.20
H3K36me3
0.20
H4K20me1
0.30
H4K20me2
0.40
H4K20me3
Vendor: RevMAb Catalog #: 31-1226-00 Lot #: R-02-01481 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0231.1
0.94
Unmodified
1.29
H3K4me1
2.93
H3K4me2
100.00
H3K4me3
0.78
H3K9me1
1.03
H3K9me2
1.05
H3K9me3
0.94
H3K27me1
0.90
H3K27me2
1.04
H3K27me3
0.90
H3K36me1
1.16
H3K36me2
0.86
H3K36me3
0.74
H4K20me1
0.83
H4K20me2
1.09
H4K20me3
Vendor: EpiCypher Catalog #: 13-0028 Lot #: 18303001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0775.1
7.00
Unmodified
8.00
H3K4me1
7.40
H3K4me2
100.00
H3K4me3
5.40
H3K9me1
5.60
H3K9me2
7.40
H3K9me3
7.00
H3K27me1
6.80
H3K27me2
7.30
H3K27me3
6.80
H3K36me1
7.10
H3K36me2
6.80
H3K36me3
5.40
H4K20me1
6.90
H4K20me2
7.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: PA5-27029 Lot #: 43727 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0193.3
0.10
Unmodified
0.60
H3K4me1
13.40
H3K4me2
100.00
H3K4me3
0.10
H3K9me1
0.20
H3K9me2
3.40
H3K9me3
0.20
H3K27me1
0.20
H3K27me2
1.60
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.30
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.70
H4K20me3
Vendor: David Bentley Catalog #: 4459 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0509.1
0.33
Unmodified
4.05
H3K4me1
37.10
H3K4me2
100.00
H3K4me3
0.30
H3K9me1
0.34
H3K9me2
1.84
H3K9me3
0.51
H3K27me1
0.43
H3K27me2
0.80
H3K27me3
0.32
H3K36me1
0.39
H3K36me2
0.30
H3K36me3
0.36
H4K20me1
0.35
H4K20me2
1.29
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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