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H3K4me1

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K4me1
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: BLR28042015-9 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.5
21.94%
5.15
Unmodified
100.00
H3K4me1
3.79
H3K4me2
4.14
H3K4me3
4.02
H3K9me1
2.13
H3K9me2
0.96
H3K9me3
2.08
H3K27me1
2.98
H3K27me2
1.29
H3K27me3
1.84
H3K36me1
3.60
H3K36me2
2.43
H3K36me3
0.64
H4K20me1
3.60
H4K20me2
5.06
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: 1992757 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.4
26.29%
5.59
Unmodified
100.00
H3K4me1
4.17
H3K4me2
4.42
H3K4me3
3.76
H3K9me1
2.30
H3K9me2
0.96
H3K9me3
2.39
H3K27me1
3.12
H3K27me2
1.50
H3K27me3
1.92
H3K36me1
3.48
H3K36me2
2.67
H3K36me3
0.85
H4K20me1
3.71
H4K20me2
5.03
H4K20me3
Vendor: EpiCypher Catalog #: 13-0040 Lot #: 19338001-42 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0784.1
26.29%
5.59
Unmodified
100.00
H3K4me1
4.17
H3K4me2
4.42
H3K4me3
3.76
H3K9me1
2.30
H3K9me2
0.96
H3K9me3
2.39
H3K27me1
3.12
H3K27me2
1.50
H3K27me3
1.92
H3K36me1
3.48
H3K36me2
2.67
H3K36me3
0.85
H4K20me1
3.71
H4K20me2
5.03
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: QL230605 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.1
35.96%
6.76
Unmodified
100.00
H3K4me1
6.32
H3K4me2
5.16
H3K4me3
4.46
H3K9me1
3.82
H3K9me2
5.68
H3K9me3
6.71
H3K27me1
3.63
H3K27me2
4.62
H3K27me3
9.37
H3K36me1
5.48
H3K36me2
5.83
H3K36me3
6.31
H4K20me1
4.31
H4K20me2
3.66
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701763 Lot #: QL230604 Clonality: Recombinant Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0645.1
30.86%
7.35
Unmodified
100.00
H3K4me1
6.90
H3K4me2
5.46
H3K4me3
16.27
H3K9me1
14.95
H3K9me2
3.90
H3K9me3
20.08
H3K27me1
6.87
H3K27me2
5.32
H3K27me3
29.82
H3K36me1
8.18
H3K36me2
21.60
H3K36me3
7.85
H4K20me1
12.54
H4K20me2
8.28
H4K20me3
Vendor: EpiCypher Catalog #: 13-0026 Lot #: 18344001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0843.1
20.42%
17.65
Unmodified
100.00
H3K4me1
9.78
H3K4me2
9.57
H3K4me3
15.24
H3K9me1
12.08
H3K9me2
12.47
H3K9me3
27.31
H3K27me1
12.17
H3K27me2
13.00
H3K27me3
22.87
H3K36me1
17.79
H3K36me2
0.00
H3K36me3
14.31
H4K20me1
14.58
H4K20me2
10.55
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: QL230605 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0234.1*
19.94%
2.57
Unmodified
100.00
H3K4me1
2.20
H3K4me2
0.78
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 5326 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 33
E0259.1*
45.67%
2.85
Unmodified
100.00
H3K4me1
2.21
H3K4me2
0.87
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 5326 Lot #: 2 Clonality: Monoclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 33
E0259.2*
33.17%
4.00
Unmodified
100.00
H3K4me1
2.48
H3K4me2
1.57
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410194 Lot #: A1862D Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 27
E0387.2*
16.18%
4.79
Unmodified
100.00
H3K4me1
5.49
H3K4me2
1.34
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Abcam Catalog #: ab8895 Lot #: GR305231-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 788
E0385.1*
63.94%
3.84
Unmodified
100.00
H3K4me1
6.33
H3K4me2
1.53
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: RevMAb Catalog #: 31-1046-00 Lot #: P-01-00415 Clonality: Monoclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0260.2*
12.73%
5.62
Unmodified
100.00
H3K4me1
5.66
H3K4me2
3.35
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720072 Lot #: RB226262 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 1
E0389.1*
40.20%
5.16
Unmodified
100.00
H3K4me1
8.29
H3K4me2
2.58
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410037 Lot #: A1657D Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 51
E0405.1*
13.55%
5.52
Unmodified
100.00
H3K4me1
8.45
H3K4me2
2.38
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EpiGentek Catalog #: A-4031-050 Lot #: 606359 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0251.1*
31.84%
3.38
Unmodified
100.00
H3K4me1
15.39
H3K4me2
1.85
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2355 Lot #: 46695 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 4
E0065.1*
42.55%
3.11
Unmodified
100.00
H3K4me1
17.39
H3K4me2
1.41
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15310037 Lot #: A399-001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 9
E0391.1*
4.41%
6.07
Unmodified
100.00
H3K4me1
5.00
H3K4me2
2.19
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410194 Lot #: A1863-001D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 27
E0387.1*
3.71%
7.28
Unmodified
100.00
H3K4me1
4.41
H3K4me2
2.27
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Active Motif Catalog #: 39297 Lot #: 21008001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 45
E0832.1*
0.72%
7.02
Unmodified
100.00
H3K4me1
6.14
H3K4me2
1.02
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EMD Millipore Catalog #: 07-436 Lot #: 2779433 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 134
E0833.1*
0.18%
9.88
Unmodified
100.00
H3K4me1
4.43
H3K4me2
0.87
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C15410194 Lot #: A1862D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 27
E0387.2
4.22%
1.45
Unmodified
100.00
H3K4me1
8.11
H3K4me2
7.11
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Active Motif Catalog #: 39297 Lot #: 1714002 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 45
E0832.2*
0.69%
19.71
Unmodified
100.00
H3K4me1
8.21
H3K4me2
4.13
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: EpiGentek Catalog #: A-4031-050 Lot #: 606359 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0251.1
55.68%
13.39
Unmodified
100.00
H3K4me1
29.22
H3K4me2
11.12
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Rockland Catalog #: 200-401-P02 Lot #: 33933 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0342.1
1.24%
57.44
Unmodified
100.00
H3K4me1
1.03
H3K4me2
0.51
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Active Motif Catalog #: 39635 Lot #: 30615011 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 7
E0834.1*
3.36%
39.47
Unmodified
100.00
H3K4me1
19.11
H3K4me2
4.60
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Rockland Catalog #: 600-401-I57 Lot #: 32389 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0340.1
1.52%
46.42
Unmodified
100.00
H3K4me1
37.85
H3K4me2
25.25
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2355 Lot #: 46694 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 4
E0065.3*
0.06%
674.79
Unmodified
100.00
H3K4me1
46.40
H3K4me2
43.26
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
No Data
H3K27me1
No Data
H3K27me2
No Data
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K4me1
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: 1992757 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.4
0.00
Unmodified
100.00
H3K4me1
0.10
H3K4me2
0.00
H3K4me3
0.00
H3K9me1
0.00
H3K9me2
0.00
H3K9me3
0.00
H3K27me1
0.00
H3K27me2
0.00
H3K27me3
0.10
H3K36me1
0.00
H3K36me2
0.00
H3K36me3
0.10
H4K20me1
0.00
H4K20me2
0.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: BLR28042015-9 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.5
0.10
Unmodified
100.00
H3K4me1
0.10
H3K4me2
0.10
H3K4me3
0.10
H3K9me1
0.10
H3K9me2
0.10
H3K9me3
0.10
H3K27me1
0.10
H3K27me2
0.10
H3K27me3
0.10
H3K36me1
0.10
H3K36me2
0.10
H3K36me3
0.10
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 701763 Lot #: QL230604 Clonality: Recombinant Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0645.1
0.10
Unmodified
100.00
H3K4me1
0.10
H3K4me2
0.00
H3K4me3
0.20
H3K9me1
0.00
H3K9me2
0.10
H3K9me3
0.30
H3K27me1
0.10
H3K27me2
0.10
H3K27me3
1.00
H3K36me1
0.10
H3K36me2
0.10
H3K36me3
2.80
H4K20me1
0.10
H4K20me2
0.10
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 710795 Lot #: QL230605 Clonality: Recombinant Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0234.1
0.60
Unmodified
100.00
H3K4me1
1.10
H3K4me2
1.40
H3K4me3
0.80
H3K9me1
0.70
H3K9me2
2.00
H3K9me3
0.70
H3K27me1
0.80
H3K27me2
1.00
H3K27me3
0.80
H3K36me1
0.80
H3K36me2
1.00
H3K36me3
0.70
H4K20me1
0.60
H4K20me2
0.60
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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