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H3K4ac

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K4ac
Vendor: EpiCypher Catalog #: 13-0034 Lot #: 19091001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0778.1
43.08%
0.11
Unmodified
100.00
H3K4ac
0.11
H3K9ac
0.08
H3K9bu
0.48
H3K9cr
0.05
H3K14ac
0.14
H3K18ac
0.10
H3K18bu
0.19
H3K18cr
107.36
tetraAc-H3
0.34
H3K23ac
0.11
H3K27ac
0.14
H3K27bu
0.07
H3K27cr
0.25
H3K27acS28ph
0.14
H3K36ac
0.51
H4K5ac
0.06
H4K8ac
0.12
H4K12ac
0.17
H4K16ac
0.09
tetraAc-H4
0.12
H4K20ac
0.29
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-24673 Lot #: UG2810492 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0652.1
87.71%
0.44
Unmodified
100.00
H3K4ac
0.26
H3K9ac
3.23
H3K9bu
0.59
H3K9cr
0.37
H3K14ac
0.50
H3K18ac
0.43
H3K18bu
0.73
H3K18cr
91.51
tetraAc-H3
0.24
H3K23ac
0.37
H3K27ac
0.56
H3K27bu
0.19
H3K27cr
0.71
H3K27acS28ph
0.77
H3K36ac
0.28
H4K5ac
0.23
H4K8ac
0.41
H4K12ac
0.39
H4K16ac
0.22
tetraAc-H4
0.44
H4K20ac
0.42
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1063-00 Lot #: Q-06-01050 Clonality: Monoclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 1
E0063.1
44.31%
0.07
Unmodified
100.00
H3K4ac
0.70
H3K9ac
0.16
H3K9bu
No Data
H3K9cr
0.15
H3K14ac
0.10
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
82.57
tetraAc-H3
No Data
H3K23ac
0.05
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
No Data
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
No Data
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K4ac
Vendor: Thermo Fisher Scientific Catalog #: MA5-24673 Lot #: UG2810492 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0652.1
0.10
Unmodified
100.00
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.10
H3K9cr
0.10
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
101.90
tetraAc-H3
0.10
H3K23ac
0.10
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.30
H3K27acS28ph
0.10
H3K36ac
0.10
H4K5ac
0.10
H4K8ac
0.10
H4K12ac
0.10
H4K16ac
0.10
tetraAc-H4
0.10
H4K20ac
0.20
tetraAc-H2A
Vendor: EpiCypher Catalog #: 13-0034 Lot #: 19091001 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0778.1
0.10
Unmodified
100.00
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.10
H3K9cr
0.10
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
54.50
tetraAc-H3
0.10
H3K23ac
0.10
H3K27ac
0.00
H3K27bu
0.10
H3K27cr
0.10
H3K27acS28ph
0.10
H3K36ac
0.10
H4K5ac
0.10
H4K8ac
0.10
H4K12ac
0.10
H4K16ac
0.10
tetraAc-H4
0.10
H4K20ac
0.10
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1063-00 Lot #: R-10-01775 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 1
E0063.2
0.00
Unmodified
100.00
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.10
H3K9cr
0.10
H3K14ac
0.00
H3K18ac
0.10
H3K18bu
0.00
H3K18cr
38.60
tetraAc-H3
0.00
H3K23ac
0.10
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.20
H3K27acS28ph
0.10
H3K36ac
0.10
H4K5ac
0.10
H4K8ac
0.10
H4K12ac
0.10
H4K16ac
0.10
tetraAc-H4
0.10
H4K20ac
0.20
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A
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