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H3K27me2

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K27me2
Vendor: Active Motif Catalog #: 61435 Lot #: 21516005 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 5
E0451.1
6.73%
8.23
Unmodified
5.68
H3K4me1
11.14
H3K4me2
8.18
H3K4me3
8.40
H3K9me1
7.57
H3K9me2
2.47
H3K9me3
32.13
H3K27me1
100.00
H3K27me2
25.05
H3K27me3
4.50
H3K36me1
10.22
H3K36me2
8.07
H3K36me3
5.28
H4K20me1
9.88
H4K20me2
10.39
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9728 Lot #: 15 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 88
E0523.1
19.42%
10.30
Unmodified
6.97
H3K4me1
12.49
H3K4me2
9.43
H3K4me3
10.99
H3K9me1
10.97
H3K9me2
3.89
H3K9me3
48.89
H3K27me1
100.00
H3K27me2
9.23
H3K27me3
6.96
H3K36me1
11.65
H3K36me2
9.91
H3K36me3
3.91
H4K20me1
9.68
H4K20me2
10.48
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11197 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0035.1
21.10%
11.28
Unmodified
8.50
H3K4me1
14.53
H3K4me2
10.17
H3K4me3
11.77
H3K9me1
12.33
H3K9me2
4.66
H3K9me3
58.72
H3K27me1
100.00
H3K27me2
10.15
H3K27me3
9.63
H3K36me1
13.03
H3K36me2
11.19
H3K36me3
4.33
H4K20me1
11.31
H4K20me2
10.86
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720098 Lot #: RF233707 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0032.1
2.42%
43.12
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
76.54
H3K27me1
100.00
H3K27me2
45.26
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: ABclonal Catalog #: A2362 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0034.1
1.73%
56.77
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
86.72
H3K27me1
100.00
H3K27me2
64.27
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Diagenode Catalog #: C1540046-50 Lot #: A1968-0042P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0619.1
2.30%
77.48
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
197.72
H3K27me1
100.00
H3K27me2
93.13
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1013 Lot #: A1968024P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0204.1
1.75%
69.69
Unmodified
No Data
H3K4me1
No Data
H3K4me2
No Data
H3K4me3
No Data
H3K9me1
No Data
H3K9me2
No Data
H3K9me3
219.96
H3K27me1
100.00
H3K27me2
108.63
H3K27me3
No Data
H3K36me1
No Data
H3K36me2
No Data
H3K36me3
No Data
H4K20me1
No Data
H4K20me2
No Data
H4K20me3
Efficiency
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-MetStat

H3K27me2
Vendor: Active Motif Catalog #: 61435 Lot #: 21516005 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 5
E0451.1
0.80
Unmodified
0.70
H3K4me1
0.90
H3K4me2
1.00
H3K4me3
1.00
H3K9me1
1.00
H3K9me2
0.80
H3K9me3
2.50
H3K27me1
100.00
H3K27me2
2.20
H3K27me3
0.80
H3K36me1
1.20
H3K36me2
0.90
H3K36me3
0.90
H4K20me1
1.00
H4K20me2
1.00
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-11197 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 0
E0035.1
1.40
Unmodified
1.30
H3K4me1
1.60
H3K4me2
1.30
H3K4me3
1.80
H3K9me1
2.30
H3K9me2
1.80
H3K9me3
3.00
H3K27me1
100.00
H3K27me2
1.40
H3K27me3
1.40
H3K36me1
2.50
H3K36me2
0.80
H3K36me3
1.80
H4K20me1
1.60
H4K20me2
1.50
H4K20me3
Vendor: Cell Signaling Technologies Catalog #: 9728 Lot #: 15 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 88
E0523.1
1.40
Unmodified
1.70
H3K4me1
2.10
H3K4me2
1.70
H3K4me3
1.90
H3K9me1
1.80
H3K9me2
1.50
H3K9me3
3.60
H3K27me1
100.00
H3K27me2
2.10
H3K27me3
1.60
H3K36me1
1.70
H3K36me2
1.90
H3K36me3
1.70
H4K20me1
1.80
H4K20me2
1.50
H4K20me3
Vendor: Abcam Catalog #: ab24684 Lot #: GR325951-1 Clonality: Polyclonal Species: Rabbit Pass/Fail: Pass # Citations: 82
E0423.2
4.50
Unmodified
2.40
H3K4me1
3.40
H3K4me2
2.40
H3K4me3
2.90
H3K9me1
6.10
H3K9me2
2.30
H3K9me3
3.40
H3K27me1
100.00
H3K27me2
2.00
H3K27me3
3.90
H3K36me1
3.30
H3K36me2
3.10
H3K36me3
3.10
H4K20me1
4.20
H4K20me2
3.40
H4K20me3
Vendor: EpiGentek Catalog #: A-4038-025 Lot #: 8060402 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0485.1
7.20
Unmodified
8.10
H3K4me1
14.20
H3K4me2
12.60
H3K4me3
9.40
H3K9me1
17.80
H3K9me2
7.80
H3K9me3
10.40
H3K27me1
100.00
H3K27me2
18.50
H3K27me3
8.80
H3K36me1
8.80
H3K36me2
7.80
H3K36me3
8.00
H4K20me1
12.10
H4K20me2
10.20
H4K20me3
Vendor: Active Motif Catalog #: 39920 Lot #: 27710001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0453.1
22.10
Unmodified
24.10
H3K4me1
48.90
H3K4me2
22.40
H3K4me3
23.10
H3K9me1
45.90
H3K9me2
24.00
H3K9me3
31.80
H3K27me1
100.00
H3K27me2
27.20
H3K27me3
18.10
H3K36me1
26.40
H3K36me2
19.60
H3K36me3
28.90
H4K20me1
120.80
H4K20me2
26.20
H4K20me3
Vendor: ABclonal Catalog #: A2362 Lot #: N/I Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0034.1
31.12
Unmodified
135.06
H3K4me1
93.76
H3K4me2
34.44
H3K4me3
32.14
H3K9me1
71.37
H3K9me2
39.82
H3K9me3
57.06
H3K27me1
100.00
H3K27me2
39.00
H3K27me3
33.62
H3K36me1
53.12
H3K36me2
35.06
H3K36me3
39.82
H4K20me1
30.50
H4K20me2
39.82
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 49-1013 Lot #: A1968024P Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0204.1
49.00
Unmodified
47.65
H3K4me1
66.17
H3K4me2
58.48
H3K4me3
58.88
H3K9me1
88.87
H3K9me2
58.43
H3K9me3
52.00
H3K27me1
100.00
H3K27me2
49.90
H3K27me3
50.95
H3K36me1
82.53
H3K36me2
28.39
H3K36me3
63.17
H4K20me1
61.58
H4K20me2
51.90
H4K20me3
Vendor: Active Motif Catalog #: 39246 Lot #: 7408001 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0452.1
44.80
Unmodified
51.90
H3K4me1
100.50
H3K4me2
44.70
H3K4me3
50.50
H3K9me1
87.00
H3K9me2
53.70
H3K9me3
63.90
H3K27me1
100.00
H3K27me2
54.70
H3K27me3
36.60
H3K36me1
51.00
H3K36me2
37.60
H3K36me3
54.80
H4K20me1
184.00
H4K20me2
49.90
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: 720098 Lot #: RF233707 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0032.1
76.80
Unmodified
100.70
H3K4me1
71.80
H3K4me2
78.90
H3K4me3
66.20
H3K9me1
114.80
H3K9me2
102.10
H3K9me3
64.80
H3K27me1
100.00
H3K27me2
77.10
H3K27me3
66.20
H3K36me1
110.20
H3K36me2
66.20
H3K36me3
73.60
H4K20me1
83.80
H4K20me2
88.70
H4K20me3
Vendor: EpiGentek Catalog #: A68392-050 Lot #: 8120342 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0493.1
89.00
Unmodified
97.00
H3K4me1
76.00
H3K4me2
85.00
H3K4me3
97.00
H3K9me1
106.00
H3K9me2
91.00
H3K9me3
84.00
H3K27me1
100.00
H3K27me2
100.00
H3K27me3
141.00
H3K36me1
226.00
H3K36me2
122.00
H3K36me3
136.00
H4K20me1
81.00
H4K20me2
109.00
H4K20me3
Vendor: Affinity Bioscience Catalog #: BF9008 Lot #: 77k1233 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0695.1
118.20
Unmodified
98.20
H3K4me1
160.00
H3K4me2
90.90
H3K4me3
70.90
H3K9me1
140.00
H3K9me2
161.80
H3K9me3
97.30
H3K27me1
100.00
H3K27me2
136.40
H3K27me3
161.80
H3K36me1
154.50
H3K36me2
205.50
H3K36me3
118.20
H4K20me1
154.50
H4K20me2
87.30
H4K20me3
H3K27me2-me3
Vendor: Abcam Catalog #: ab6147 Lot #: GR232895-13 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 23
E0424.1
2.40
Unmodified
2.10
H3K4me1
2.30
H3K4me2
2.40
H3K4me3
5.10
H3K9me1
3.70
H3K9me2
4.50
H3K9me3
3.50
H3K27me1
15.00
H3K27me2
100.00
H3K27me3
3.30
H3K36me1
4.10
H3K36me2
3.90
H3K36me3
5.30
H4K20me1
3.40
H4K20me2
4.20
H4K20me3
Vendor: Thermo Fisher Scientific Catalog #: MA5-23511 Lot #: UH2819271 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0667.1
5.10
Unmodified
6.80
H3K4me1
6.40
H3K4me2
5.20
H3K4me3
4.80
H3K9me1
4.70
H3K9me2
5.40
H3K9me3
22.50
H3K27me1
100.00
H3K27me2
95.00
H3K27me3
5.50
H3K36me1
6.00
H3K36me2
5.80
H3K36me3
5.70
H4K20me1
5.40
H4K20me2
4.50
H4K20me3
Unmodified
H3K4me1
H3K4me2
H3K4me3
H3K9me1
H3K9me2
H3K9me3
H3K27me1
H3K27me2
H3K27me3
H3K36me1
H3K36me2
H3K36me3
H4K20me1
H4K20me2
H4K20me3
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