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H4K16

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H4K16ac
Vendor: Thermo Fisher Scientific Catalog #: MA5-27794 Lot #: UG2799525H Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0640.1
5.24%
7.92
Unmodified
7.40
H3K4ac
5.51
H3K9ac
8.46
H3K9bu
10.69
H3K9cr
7.15
H3K14ac
8.74
H3K18ac
6.81
H3K18bu
8.41
H3K18cr
8.44
tetraAc-H3
9.86
H3K23ac
8.46
H3K27ac
12.10
H3K27bu
5.05
H3K27cr
13.42
H3K27acS28ph
7.93
H3K36ac
8.81
H4K5ac
3.76
H4K8ac
7.91
H4K12ac
100.00
H4K16ac
119.44
tetraAc-H4
8.82
H4K20ac
17.53
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1083-00 Lot #: S-01-01895 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0115.2
0.03%
21.72
Unmodified
29.84
H3K4ac
48.37
H3K9ac
21.70
H3K9bu
25.18
H3K9cr
42.18
H3K14ac
81.63
H3K18ac
23.64
H3K18bu
14.14
H3K18cr
12.09
tetraAc-H3
36.94
H3K23ac
29.52
H3K27ac
58.92
H3K27bu
19.51
H3K27cr
5.21
H3K27acS28ph
22.73
H3K36ac
28.61
H4K5ac
11.69
H4K8ac
33.16
H4K12ac
100.00
H4K16ac
751.06
tetraAc-H4
0.00
H4K20ac
159.05
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1083-00 Lot #: P-01-00399 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0115.1
0.07%
49.24
Unmodified
962.12
H3K4ac
2740.92
H3K9ac
12.33
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
No Data
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
24.74
tetraAc-H3
No Data
H3K23ac
10.63
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
93.21
H4K8ac
No Data
H4K12ac
100.00
H4K16ac
252.63
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720083 Lot #: QL226264 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0114.1
0.07%
71.59
Unmodified
455.01
H3K4ac
1707.26
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
No Data
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
31.70
tetraAc-H3
No Data
H3K23ac
74.15
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
34.78
H4K8ac
No Data
H4K12ac
100.00
H4K16ac
92.98
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: ABclonal Catalog #: A5280 Lot #: 2155000401 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0181.1
0.11%
18.43
Unmodified
1109.80
H3K4ac
2433.90
H3K9ac
6.55
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
No Data
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
19.60
tetraAc-H3
No Data
H3K23ac
5.73
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
92.27
H4K8ac
No Data
H4K12ac
100.00
H4K16ac
14.86
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
tetraAc-H4
Vendor: Thermo Fisher Scientific Catalog #: PA1-84526 Lot #: UG2815638 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0703.1
0.14%
0.69
Unmodified
No Data
H3K4ac
No Data
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
No Data
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
No Data
tetraAc-H3
No Data
H3K23ac
No Data
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
11.58
H4K5ac
4.80
H4K8ac
23.15
H4K12ac
0.65
H4K16ac
100.00
tetraAc-H4
3.23
H4K20ac
28.36
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H4K16ac
Vendor: Thermo Fisher Scientific Catalog #: MA5-27794 Lot #: UG2799525H Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 0
E0640.1
0.00
Unmodified
0.10
H3K4ac
0.10
H3K9ac
0.00
H3K9bu
0.00
H3K9cr
0.00
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
0.10
tetraAc-H3
0.10
H3K23ac
0.10
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.00
H3K27acS28ph
0.00
H3K36ac
0.00
H4K5ac
0.00
H4K8ac
0.10
H4K12ac
100.00
H4K16ac
55.90
tetraAc-H4
0.10
H4K20ac
0.00
tetraAc-H2A
Vendor: Abcam Catalog #: ab109463 Lot #: GR3178065-11 Clonality: Monoclonal Species: Rabbit Pass/Fail: Pass # Citations: 38
E0554.1
0.60
Unmodified
0.40
H3K4ac
0.60
H3K9ac
0.50
H3K9bu
0.60
H3K9cr
0.70
H3K14ac
0.50
H3K18ac
0.50
H3K18bu
0.60
H3K18cr
0.40
tetraAc-H3
0.40
H3K23ac
0.40
H3K27ac
0.40
H3K27bu
0.40
H3K27cr
0.40
H3K27acS28ph
0.60
H3K36ac
7.20
H4K5ac
0.50
H4K8ac
0.50
H4K12ac
100.00
H4K16ac
355.30
tetraAc-H4
0.40
H4K20ac
1.10
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1083-00 Lot #: S-01-01895 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0115.2
11.70
Unmodified
11.60
H3K4ac
13.00
H3K9ac
11.60
H3K9bu
13.70
H3K9cr
14.20
H3K14ac
9.70
H3K18ac
10.10
H3K18bu
9.10
H3K18cr
12.40
tetraAc-H3
10.30
H3K23ac
10.30
H3K27ac
11.70
H3K27bu
13.40
H3K27cr
10.80
H3K27acS28ph
12.40
H3K36ac
17.40
H4K5ac
13.60
H4K8ac
19.20
H4K12ac
100.00
H4K16ac
651.00
tetraAc-H4
20.80
H4K20ac
12.10
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1083-00 Lot #: P-01-00399 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0115.1
11.80
Unmodified
12.00
H3K4ac
12.60
H3K9ac
12.30
H3K9bu
16.00
H3K9cr
18.60
H3K14ac
10.70
H3K18ac
13.60
H3K18bu
11.50
H3K18cr
13.00
tetraAc-H3
11.00
H3K23ac
13.10
H3K27ac
12.10
H3K27bu
12.60
H3K27cr
11.80
H3K27acS28ph
13.50
H3K36ac
17.00
H4K5ac
13.00
H4K8ac
18.90
H4K12ac
100.00
H4K16ac
500.50
tetraAc-H4
20.10
H4K20ac
13.60
tetraAc-H2A
Vendor: ABclonal Catalog #: A5280 Lot #: 2155000401 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0181.1
14.80
Unmodified
16.50
H3K4ac
17.90
H3K9ac
15.80
H3K9bu
17.30
H3K9cr
19.10
H3K14ac
17.70
H3K18ac
18.80
H3K18bu
17.90
H3K18cr
23.40
tetraAc-H3
21.10
H3K23ac
21.70
H3K27ac
20.10
H3K27bu
19.10
H3K27cr
24.50
H3K27acS28ph
18.80
H3K36ac
23.10
H4K5ac
27.40
H4K8ac
31.20
H4K12ac
100.00
H4K16ac
99.60
tetraAc-H4
26.80
H4K20ac
32.00
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720083 Lot #: 2149416 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0114.2
22.00
Unmodified
24.70
H3K4ac
21.80
H3K9ac
18.40
H3K9bu
20.70
H3K9cr
20.60
H3K14ac
23.40
H3K18ac
24.30
H3K18bu
22.80
H3K18cr
26.00
tetraAc-H3
22.40
H3K23ac
19.50
H3K27ac
15.10
H3K27bu
16.90
H3K27cr
13.40
H3K27acS28ph
17.30
H3K36ac
15.60
H4K5ac
11.70
H4K8ac
12.90
H4K12ac
100.00
H4K16ac
50.80
tetraAc-H4
9.20
H4K20ac
18.70
tetraAc-H2A
Vendor: Active Motif Catalog #: 39167 Lot #: 33311003 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 43
E0506.1
34.00
Unmodified
46.50
H3K4ac
44.90
H3K9ac
37.40
H3K9bu
40.90
H3K9cr
46.90
H3K14ac
43.50
H3K18ac
45.40
H3K18bu
43.60
H3K18cr
56.90
tetraAc-H3
55.50
H3K23ac
50.90
H3K27ac
48.00
H3K27bu
47.30
H3K27cr
58.10
H3K27acS28ph
48.20
H3K36ac
70.20
H4K5ac
68.70
H4K8ac
87.10
H4K12ac
100.00
H4K16ac
194.30
tetraAc-H4
64.80
H4K20ac
92.00
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720083 Lot #: QL226264 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0114.1
56.70
Unmodified
65.80
H3K4ac
83.00
H3K9ac
80.70
H3K9bu
68.70
H3K9cr
70.60
H3K14ac
64.80
H3K18ac
79.70
H3K18bu
71.40
H3K18cr
98.30
tetraAc-H3
73.30
H3K23ac
92.50
H3K27ac
82.20
H3K27bu
119.70
H3K27cr
109.20
H3K27acS28ph
90.80
H3K36ac
84.20
H4K5ac
81.90
H4K8ac
88.60
H4K12ac
100.00
H4K16ac
113.90
tetraAc-H4
93.70
H4K20ac
127.50
tetraAc-H2A
tetraAc-H4
Vendor: EMD Millipore Catalog #: 05-1355 Lot #: 2854397 Clonality: Monoclonal Species: Mouse Pass/Fail: Pass # Citations: 8
E0282.1
0.10
Unmodified
0.10
H3K4ac
0.10
H3K9ac
0.10
H3K9bu
0.10
H3K9cr
0.10
H3K14ac
0.10
H3K18ac
0.10
H3K18bu
0.10
H3K18cr
0.10
tetraAc-H3
0.10
H3K23ac
0.10
H3K27ac
0.10
H3K27bu
0.10
H3K27cr
0.10
H3K27acS28ph
0.10
H3K36ac
0.10
H4K5ac
0.10
H4K8ac
94.00
H4K12ac
0.10
H4K16ac
100.00
tetraAc-H4
0.10
H4K20ac
0.20
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: PA1-84526 Lot #: UG2815638 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0703.1
0.60
Unmodified
0.60
H3K4ac
0.60
H3K9ac
0.70
H3K9bu
0.70
H3K9cr
0.80
H3K14ac
0.70
H3K18ac
0.70
H3K18bu
0.70
H3K18cr
1.00
tetraAc-H3
0.80
H3K23ac
1.00
H3K27ac
1.10
H3K27bu
1.20
H3K27cr
1.10
H3K27acS28ph
0.60
H3K36ac
8.20
H4K5ac
26.10
H4K8ac
42.50
H4K12ac
0.90
H4K16ac
100.00
tetraAc-H4
1.20
H4K20ac
3.00
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA3-066 Lot #: TE271086 Clonality: Monoclonal Species: Mouse Pass/Fail: Fail # Citations: 0
E0702.1
1.50
Unmodified
1.60
H3K4ac
2.10
H3K9ac
1.80
H3K9bu
1.70
H3K9cr
9.80
H3K14ac
2.00
H3K18ac
1.50
H3K18bu
1.40
H3K18cr
36.60
tetraAc-H3
2.60
H3K23ac
3.70
H3K27ac
1.70
H3K27bu
2.30
H3K27cr
1.60
H3K27acS28ph
1.50
H3K36ac
14.40
H4K5ac
32.10
H4K8ac
19.50
H4K12ac
3.00
H4K16ac
100.00
tetraAc-H4
3.30
H4K20ac
19.10
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: PA5-40084 Lot #: UH2813212 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 1
E0701.1
9.90
Unmodified
7.40
H3K4ac
8.30
H3K9ac
10.30
H3K9bu
11.20
H3K9cr
15.70
H3K14ac
9.20
H3K18ac
8.90
H3K18bu
8.40
H3K18cr
21.20
tetraAc-H3
10.80
H3K23ac
16.60
H3K27ac
13.00
H3K27bu
14.20
H3K27cr
22.10
H3K27acS28ph
8.00
H3K36ac
23.50
H4K5ac
17.80
H4K8ac
75.20
H4K12ac
13.40
H4K16ac
100.00
tetraAc-H4
17.70
H4K20ac
51.50
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A
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