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H3K18

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SNAP-ChIP Luminex

For final validation, we test each antibody using SNAP-ChIP® (Sample Normalization & Antibody Profiling for ChIP) Spike-in Control Panels added to a ChIP experiment. Passing requires that an antibody display <20% cross-reactivity (relative to on-target binding) and >5% enrichment of on-target PTM vs. Input.

Heatmaps are sorted by PTM, and are structured such that the most specific antibodies that passed testing against the full SNAP-ChIP spike-in panel are ranked at the top. These antibodies are recommended for ChIP-seq. The remaining antibodies in the heatmap are separated into three groups: those that failed testing against the full SNAP-ChIP panel, antibodies that were only tested against a subset of spike-ins and passed, and those tested against a subset of spike-ins that failed. Within each subgroup, antibodies are ranked by specificity.

* Denotes antibodies that were tested in Shah et al. 2018.

Efficiency (% Recovered)

< 5% 5-10% > 10%

Efficiency, or enrichment, is the percent recovery of on-target PTM compared to input. Efficiency scores are binned into three categories, shown on the scale. >5% efficiency is required for SNAP-ChIP Certification.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K18ac
Vendor: Cell Signaling Technologies Catalog #: 13998 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 12
E0475.1
40.69%
9.57
Unmodified
1.76
H3K4ac
1.15
H3K9ac
1.73
H3K9bu
1.51
H3K9cr
1.55
H3K14ac
100.00
H3K18ac
2.42
H3K18bu
2.20
H3K18cr
83.77
tetraAc-H3
1.89
H3K23ac
1.64
H3K27ac
1.84
H3K27bu
2.85
H3K27cr
7.46
H3K27acS28ph
2.14
H3K36ac
2.85
H4K5ac
1.19
H4K8ac
1.38
H4K12ac
1.60
H4K16ac
2.03
tetraAc-H4
39.34
H4K20ac
2.91
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: PA5-85523 Lot #: UH2819165E Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0639.2
35.62%
7.83
Unmodified
3.61
H3K4ac
2.82
H3K9ac
2.03
H3K9bu
1.01
H3K9cr
0.84
H3K14ac
100.00
H3K18ac
85.93
H3K18bu
51.11
H3K18cr
103.60
tetraAc-H3
8.50
H3K23ac
1.45
H3K27ac
1.90
H3K27bu
1.91
H3K27cr
8.50
H3K27acS28ph
1.75
H3K36ac
3.25
H4K5ac
0.95
H4K8ac
0.99
H4K12ac
1.03
H4K16ac
1.57
tetraAc-H4
2.96
H4K20ac
2.04
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: PA5-85523 Lot #: UG2799523D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0639.1
43.07%
8.68
Unmodified
3.58
H3K4ac
2.14
H3K9ac
2.31
H3K9bu
1.14
H3K9cr
1.08
H3K14ac
100.00
H3K18ac
103.37
H3K18bu
55.72
H3K18cr
115.48
tetraAc-H3
9.49
H3K23ac
1.86
H3K27ac
2.42
H3K27bu
2.24
H3K27cr
9.90
H3K27acS28ph
2.01
H3K36ac
3.48
H4K5ac
0.93
H4K8ac
1.08
H4K12ac
1.20
H4K16ac
1.74
tetraAc-H4
1.75
H4K20ac
2.07
tetraAc-H2A
Vendor: Cell Signaling Technologies Catalog #: 9675 Lot #: 2 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 70
E0309.1
12.69%
6.37
Unmodified
2.30
H3K4ac
3.96
H3K9ac
No Data
H3K9bu
2.13
H3K9cr
0.76
H3K14ac
100.00
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
182.03
tetraAc-H3
No Data
H3K23ac
1.87
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
0.37
H4K8ac
No Data
H4K12ac
1.03
H4K16ac
0.42
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: ABclonal Catalog #: A7257 Lot #: 2300070401 Clonality: Polyclonal Species: Rabbit Pass/Fail: Limited Data # Citations: 0
E0180.1
38.58%
8.73
Unmodified
1.66
H3K4ac
8.22
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
0.91
H3K14ac
100.00
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
124.34
tetraAc-H3
No Data
H3K23ac
5.88
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
1.64
H4K8ac
No Data
H4K12ac
0.98
H4K16ac
0.69
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720095 Lot #: RB226266 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0021.1
4.60%
18.60
Unmodified
No Data
H3K4ac
42.90
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
4.81
H3K14ac
100.00
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
266.07
tetraAc-H3
No Data
H3K23ac
6.65
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
2.61
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
7.26
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-24669 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0025.1
1.90%
10.27
Unmodified
No Data
H3K4ac
178.35
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
11.38
H3K14ac
100.00
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
677.27
tetraAc-H3
No Data
H3K23ac
5.16
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
13.35
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
18.53
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1055-00 Lot #: P-01-00408 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0022.1
1.77%
10.05
Unmodified
No Data
H3K4ac
203.50
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
10.71
H3K14ac
100.00
H3K18ac
No Data
H3K18bu
No Data
H3K18cr
683.80
tetraAc-H3
No Data
H3K23ac
3.88
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
9.78
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
13.86
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
H3K18cr
Vendor: Thermo Fisher Scientific Catalog #: 703472 Lot #: N/I Clonality: Recombinant Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0593.1
63.98%
3.82
Unmodified
No Data
H3K4ac
No Data
H3K9ac
No Data
H3K9bu
No Data
H3K9cr
No Data
H3K14ac
27.28
H3K18ac
126.10
H3K18bu
100.00
H3K18cr
100.00
tetraAc-H3
No Data
H3K23ac
No Data
H3K27ac
No Data
H3K27bu
No Data
H3K27cr
No Data
H3K27acS28ph
No Data
H3K36ac
No Data
H4K5ac
No Data
H4K8ac
No Data
H4K12ac
No Data
H4K16ac
No Data
tetraAc-H4
No Data
H4K20ac
No Data
tetraAc-H2A
Efficiency
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A

We use Luminex® multiplexing technology to screen antibody candidates against modified nucleosome substrates. To pass this triage experiment, antibodies must display <10% cross-reactivity relative to the on-target PTM.

Antibodies are sorted in the heatmap by PTM and then by overall specificity, with antibodies that passed Luminex and have highest specificity ranked at the top. Note that passing Luminex does not guarantee an antibody will work well in ChIP experiments; so be sure to confirm that your antibody also passes testing against SNAP-ChIP spike-ins.

Specificity (% On-Target)

0 20 40 60 80 100 120

Antibody binding is expressed relative to the on-target PTM. Binding is presented as a gradient from blue (weak activity) to red (strong activity). Increasing red across the entire panel denotes antibodies with high cross-reactivity.

K-AcylStat

H3K18ac
Vendor: Thermo Fisher Scientific Catalog #: PA5-85523 Lot #: UG2799523D Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0639.1
0.20
Unmodified
0.30
H3K4ac
1.10
H3K9ac
0.30
H3K9bu
0.20
H3K9cr
0.20
H3K14ac
100.00
H3K18ac
48.50
H3K18bu
28.90
H3K18cr
145.60
tetraAc-H3
4.00
H3K23ac
0.40
H3K27ac
0.30
H3K27bu
0.30
H3K27cr
0.50
H3K27acS28ph
0.20
H3K36ac
0.30
H4K5ac
0.30
H4K8ac
0.40
H4K12ac
0.30
H4K16ac
0.50
tetraAc-H4
8.50
H4K20ac
0.30
tetraAc-H2A
Vendor: Cell Signaling Technologies Catalog #: 13998 Lot #: 1 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 12
E0475.1
0.30
Unmodified
0.30
H3K4ac
0.40
H3K9ac
0.50
H3K9bu
0.40
H3K9cr
0.50
H3K14ac
100.00
H3K18ac
0.50
H3K18bu
0.40
H3K18cr
157.10
tetraAc-H3
0.30
H3K23ac
0.50
H3K27ac
0.30
H3K27bu
0.40
H3K27cr
0.60
H3K27acS28ph
0.30
H3K36ac
0.40
H4K5ac
0.60
H4K8ac
0.40
H4K12ac
0.30
H4K16ac
0.60
tetraAc-H4
111.80
H4K20ac
0.30
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: PA5-85523 Lot #: UH2819165E Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0639.2
0.20
Unmodified
0.30
H3K4ac
3.60
H3K9ac
0.30
H3K9bu
0.20
H3K9cr
0.20
H3K14ac
100.00
H3K18ac
45.40
H3K18bu
27.10
H3K18cr
148.00
tetraAc-H3
6.40
H3K23ac
0.40
H3K27ac
0.30
H3K27bu
0.30
H3K27cr
0.60
H3K27acS28ph
0.20
H3K36ac
0.20
H4K5ac
0.30
H4K8ac
0.30
H4K12ac
0.20
H4K16ac
0.50
tetraAc-H4
34.40
H4K20ac
0.40
tetraAc-H2A
Vendor: Cell Signaling Technologies Catalog #: 9675 Lot #: 2 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 70
E0309.1
1.70
Unmodified
2.10
H3K4ac
2.40
H3K9ac
2.00
H3K9bu
1.90
H3K9cr
2.40
H3K14ac
100.00
H3K18ac
54.90
H3K18bu
17.60
H3K18cr
171.60
tetraAc-H3
2.10
H3K23ac
5.80
H3K27ac
3.20
H3K27bu
2.30
H3K27cr
11.30
H3K27acS28ph
2.30
H3K36ac
3.30
H4K5ac
2.90
H4K8ac
3.20
H4K12ac
3.10
H4K16ac
4.10
tetraAc-H4
7.60
H4K20ac
5.60
tetraAc-H2A
Vendor: EpiGentek Catalog #: C10010-1 Lot #: 3K18A Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0488.1
0.40
Unmodified
0.70
H3K4ac
2.80
H3K9ac
1.10
H3K9bu
0.80
H3K9cr
0.60
H3K14ac
100.00
H3K18ac
69.20
H3K18bu
28.10
H3K18cr
160.10
tetraAc-H3
0.60
H3K23ac
8.50
H3K27ac
1.00
H3K27bu
0.60
H3K27cr
4.60
H3K27acS28ph
0.50
H3K36ac
0.60
H4K5ac
0.90
H4K8ac
0.80
H4K12ac
0.70
H4K16ac
1.20
tetraAc-H4
11.90
H4K20ac
0.80
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: MA5-24669 Lot #: N/I Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0025.1
2.50
Unmodified
2.60
H3K4ac
3.50
H3K9ac
2.20
H3K9bu
2.70
H3K9cr
4.60
H3K14ac
100.00
H3K18ac
3.30
H3K18bu
3.00
H3K18cr
490.50
tetraAc-H3
3.30
H3K23ac
4.60
H3K27ac
2.30
H3K27bu
2.20
H3K27cr
4.90
H3K27acS28ph
3.30
H3K36ac
9.00
H4K5ac
23.70
H4K8ac
29.90
H4K12ac
29.40
H4K16ac
47.80
tetraAc-H4
138.70
H4K20ac
10.20
tetraAc-H2A
Vendor: RevMAb Catalog #: 31-1055-00 Lot #: P-01-00408 Clonality: Monoclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0022.1
2.40
Unmodified
2.70
H3K4ac
2.70
H3K9ac
2.20
H3K9bu
2.50
H3K9cr
4.10
H3K14ac
100.00
H3K18ac
3.50
H3K18bu
2.70
H3K18cr
500.20
tetraAc-H3
3.10
H3K23ac
4.90
H3K27ac
2.30
H3K27bu
2.50
H3K27cr
4.00
H3K27acS28ph
3.20
H3K36ac
8.60
H4K5ac
23.20
H4K8ac
30.30
H4K12ac
28.10
H4K16ac
51.10
tetraAc-H4
159.30
H4K20ac
9.70
tetraAc-H2A
Vendor: ABclonal Catalog #: A7257 Lot #: 2300070401 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0180.1
1.00
Unmodified
1.20
H3K4ac
1.80
H3K9ac
1.40
H3K9bu
1.50
H3K9cr
1.50
H3K14ac
100.00
H3K18ac
131.00
H3K18bu
35.10
H3K18cr
183.00
tetraAc-H3
1.10
H3K23ac
14.60
H3K27ac
1.10
H3K27bu
1.40
H3K27cr
4.20
H3K27acS28ph
1.10
H3K36ac
1.60
H4K5ac
3.80
H4K8ac
1.50
H4K12ac
1.50
H4K16ac
3.70
tetraAc-H4
141.40
H4K20ac
2.20
tetraAc-H2A
Vendor: Thermo Fisher Scientific Catalog #: 720095 Lot #: RB226266 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0021.1
21.10
Unmodified
26.00
H3K4ac
32.80
H3K9ac
30.60
H3K9bu
28.10
H3K9cr
26.60
H3K14ac
100.00
H3K18ac
460.60
H3K18bu
171.80
H3K18cr
201.90
tetraAc-H3
30.70
H3K23ac
44.60
H3K27ac
32.80
H3K27bu
45.50
H3K27cr
42.40
H3K27acS28ph
30.90
H3K36ac
31.50
H4K5ac
36.30
H4K8ac
43.10
H4K12ac
34.10
H4K16ac
48.00
tetraAc-H4
47.30
H4K20ac
52.20
tetraAc-H2A
H3K18bu
Vendor: PTMBioLabs Catalog #: PTM-306 Lot #: ZD036G519P4 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 0
E0215.1
0.20
Unmodified
0.40
H3K4ac
0.40
H3K9ac
13.60
H3K9bu
7.80
H3K9cr
0.20
H3K14ac
2.60
H3K18ac
100.00
H3K18bu
57.80
H3K18cr
2.90
tetraAc-H3
0.40
H3K23ac
0.50
H3K27ac
8.20
H3K27bu
3.80
H3K27cr
0.20
H3K27acS28ph
0.30
H3K36ac
0.20
H4K5ac
0.20
H4K8ac
0.30
H4K12ac
0.20
H4K16ac
0.30
tetraAc-H4
0.50
H4K20ac
1.70
tetraAc-H2A
H3K18cr
Vendor: PTM BioLabs Catalog #: PTM-517 Lot #: ZT035I118P0 Clonality: Polyclonal Species: Rabbit Pass/Fail: Fail # Citations: 2
E0213.1
0.10
Unmodified
0.10
H3K4ac
0.20
H3K9ac
0.20
H3K9bu
0.60
H3K9cr
0.20
H3K14ac
1.80
H3K18ac
38.50
H3K18bu
100.00
H3K18cr
0.50
tetraAc-H3
0.10
H3K23ac
0.20
H3K27ac
0.10
H3K27bu
0.40
H3K27cr
0.10
H3K27acS28ph
0.10
H3K36ac
0.20
H4K5ac
0.10
H4K8ac
0.10
H4K12ac
0.10
H4K16ac
0.20
tetraAc-H4
0.20
H4K20ac
0.10
tetraAc-H2A
Unmodified
H3K4ac
H3K9ac
H3K9bu
H3K9cr
H3K14ac
H3K18ac
H3K18bu
H3K18cr
tetraAc-H3
H3K23ac
H3K27ac
H3K27bu
H3K27cr
H3K27acS28ph
H3K36ac
H4K5ac
H4K8ac
H4K12ac
H4K16ac
tetraAc-H4
H4K20ac
tetraAc-H2A
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